Celigo Archives | Page 9 of 10 | Nexcelom Bioscience

Celigo assists in optimizing CHO cells for biopharmaceutical production

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]

By Polly Mitchell|2021-06-15T19:13:58+00:00February 11th, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: 96-well plates, Celigo, Cell Counting, CHO, CHO cells, Hoechst, Novo Nordisk, Propidium Iodide|0 Comments

Celigo evaluates quinomycin A as a possible therapeutic tool for pancreatic cancer

The Notch-disrupting and cancer stem cell-inhibiting effects of the drug quinomycin A were investigated at the University of Kansas Medical Center. Using human pancreatic cancer cells PanC-1, MiaPaCa-2, and BxPC-3 and the Celigo to determine the number and size of pancreatospheres, researchers evaluated the drug’s ability to block cancer stem cell growth via inhibition of the Notch signaling pathway. After administration of the drug, proliferation and colony formation were blocked in cancer cell lines but not in normal pancreatic epithelial cells. Furthermore, cancer stem cell markers were reduced as was pancreatosphere formation. This work identifies quinomycin A as an efficacious [...]

By Polly Mitchell|2021-06-15T19:15:28+00:00January 26th, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: Cancer Cell Lines, Celigo|0 Comments

Celigo furthers studies of homologous recombination DNA repair machinery

The Danish Cancer Society Research Center recently published a study furthering their analysis of homologous recombination DNA repair machinery. The group previously reported on a growth factor, PSIP1, that enables DNA end resection. With GFP-transfected U2OS cells, the group investigated a structurally similar protein, hepatoma-derived growth factor-related protein 2 (HDGFRP2). The Celigo analyzed cell number and viability via fluorescent markers. The group reports that HDGRFP2 may help to repair silent genes that have been impaired or active genes inhibited by DNA damage. Read the full publication here.

By Polly Mitchell|2021-06-15T19:15:44+00:00January 21st, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: Celigo, Image Cytometer, image cytometry|0 Comments

Celigo evaluates a plant extract for glioblastomoa multiforme treatment

At the Canary Center at Stanford for Early Cancer Detection, investigators studied how AshwaMAX (a steroidal lactone from a winter cherry plant, Withania somnifera, extract) might work as an oral treatment for those with the highly aggressive cancer glioblastoma multiforme (GBM). A heterogeneous disease, non-specific therapies for GBM have proven largely ineffective. Two patient-derived GBM lines (GBM2, GBM39) and one GBM cell line were cultured to create neurospheres that were then exposed to various concentrations of AshwaMAX. Celigo measured cell proliferation and cell death via Trypan Blue staining. AshwaMAX inhibited the neurospheres at nanomolar concentrations. After additional work in vivo, [...]

By Polly Mitchell|2021-06-15T19:44:29+00:00December 1st, 2015|Categories: Celigo, Celigo User Publications, Instrument|Tags: Cancer Cell Lines, Celigo, cell death, Cell Proliferation, Image Cytometer, image cytometry, Imaging Cytometer, Imaging Cytometry, Trypan Blue|0 Comments

Celigo Advances studies in T Cell Therapy as a pediatric CHOP patient defeats her recurrent leukemia with immunotherapy

Emily Whitehead, cancer survivor, holding Nexcelom cell counting sheep. Thanks to the lifesaving T cell therapy clinical trial at Children’s Hospital of Philadelphia, Emily Whitehead is now two years cancer free. Diagnosed with acute lymphoblastic leukemia (ALL) right after her 5th birthday, doctors discovered that Emily’s leukemia was particularly resistant to chemotherapy, as are roughly 15% of the total number of ALL cases. After two recurrences of the disease, Emily’s parents enrolled her in a clinical trial for CTL019, an experimental therapeutic using a patient’s own reprogrammed T cells to eliminate the cancer cells. Now 9 years old, [...]

By Polly Mitchell|2021-06-15T19:45:08+00:00November 20th, 2015|Categories: CAR T / Cell Therapy, Celigo, Celigo Application News, Instrument|Tags: Cancer Cell Lines, Celigo, Image Cytometer, image cytometry, leukemia, T cell therapy, T cells|0 Comments

96- and 384-well Ultra-Low Adhesion Round-Bottom Multi-Well Plates now Available

384-well Ultra-low attachment treatedround bottom multi-well plates 96-well Ultra-low attachment treated round bottom multi-well plates Nexcelom has just released two new products: Ultra-low adhesion round-bottom plates in 96-well and 384-well formats that are specifically designed for working with 3D tumor spheroids. These clear wall, clear bottom plates were validated with U87 MG cells and tracked using a bright-field imaging technique on the Celigo Image Cytometer. These plates are ideal for single spheroid drug screening assays and can also be used to investigate spheroid invasion with the addition of Matrigel. You can read more about the plates [...]

By Polly Mitchell|2021-06-15T19:47:30+00:00October 27th, 2015|Categories: Cellometer, Cellometer Product News, Instrument|Tags: 384-well plates, 3D Tumor Spheroids, 96-well plates, Celigo, drug screening|0 Comments

Ignyta tests Celigo against Cell Titer-Glo for cell proliferation

Here's a great example of how the Celigo image cytometer is able to perform common experiments while saving time and money! Ignyta, Inc. was looking for a new way to perform reagent-free proliferation analyses with suspension cells. This new method had to produce results which correlated well to their current method, Cell Titer-Glo®. Nexcelom and Ignyta partnered to perform a head-to-head cell proliferation comparison between Celigo® and Cell Titer-Glo. Using four suspension cell types (Ba/F3 parental cell line, Ba/F3 expressing an oncogenic gene, oncogenic gene mutant A and B), Ignyta plated all cells at a concentration of 5,000 cells/well in [...]

By Polly Mitchell|2021-06-15T19:47:39+00:00October 23rd, 2015|Categories: Celigo, Celigo User Publications, Instrument|Tags: Celigo, Cell Proliferation, Image Cytometer|0 Comments

Live Webinar! Join us for “3D Tumor Spheroids Based Functional Assays”

Recent publications have suggested that using 3D tumor spheroids is a more predictive model for preclinical research. Nexcelom Bioscience has developed a standardized microplate method for rapid generation, imaging and analysis of 3D tumor spheroids using the Celigo image cytometer. 40 cancer cell lines' ability to form spheroids, optimal seeding densities and culture conditions have been established. Protocols measuring tumor growth, viability, migration and invasion have been utilized by many researchers for routine preclinical drug studies. If you are interested to learn more about how your research might benefit from working with 3D tumor spheroids, this is a great place to start! [...]

By Polly Mitchell|2021-06-15T19:48:33+00:00October 13th, 2015|Categories: 3D Models, Celigo, Celigo Application News, Instrument|Tags: 3D Tumor Spheroids, Celigo, webinar|0 Comments

Nexcelom’s Celigo Detects CRISPR Transfection Efficiency of sgRNA in CHO Cells
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Nexcelom’s Celigo Detects CRISPR Transfection Efficiency of sgRNA in CHO Cells

Celigo, Celigo User Publications, Instrument

Nexcelom’s Celigo Detects CRISPR Transfection Efficiency of sgRNA in CHO Cells

Novel web-based tool ensures efficient, fast, and low-cost genetic manipulation of CHO cells for future biopharmaceutical applications.

By Leo Chan|2021-06-15T19:52:16+00:00March 30th, 2015|Categories: Celigo, Celigo User Publications, Instrument|Tags: Celigo, CHO, CRISPR, Image Cytometer, transfection efficiency|0 Comments

Celigo User Training Webinar : 11/13/14

Nexcelom will be hosting the next Celigo User Training Webinar on Thursday November 13th at 1:00 ET. This webinar will be recorded and the recording will be available after the session concludes. Our Applications Scientist, Sarah Kessel will be presenting this webinar on the topic of: Measuring Transfection Efficiency on your Celigo This webinar will specifically cover: Celigo Image Cytometer Label-free monitoring of your cell transfection efficiency over time using cell counts or confluence Measuring transfection efficiency, where total cell counts are obtained with Hoechst stain Time for questions and answers You can Register Here for this [...]

By Polly Mitchell|2014-11-06T12:33:24+00:00November 6th, 2014|Categories: General Discussions|Tags: Celigo, training, transfection efficiency, webinar|0 Comments