Exploring Long-term Protection of Normal Human Fibroblasts and Epithelial Cells from Chemotherapy in Cell Culture

Exploring Long-term Protection of Normal Human Fibroblasts and Epithelial Cells from Chemotherapy in Cell Culture Authors: Pasha Apontes, Olga V. Leontieva, Zoya N. Demidenko, Fengzhi Li, Mikhail V. BlagosklonnyOncotarget. 2011 March 2(3): 222-233; PMCID: PMC3260824 Background: Microtubule-targeting agents or mitotic inhibitors are commonly used in chemotherapy for cancer. These agents kill proliferating, or cycling, cells by causing fatal mitotic arrest. Because many normal cells cycle very frequently (bone marrow cells, hair follicles, mucosal cells, endothelial cells), these normal cells are also affected by chemotherapy, leading to many side effects and reduced chemotherapy treatment. […]

By |2021-06-15T20:53:30+00:00March 28th, 2012|Categories: Immuno-oncology, Publication Review|1 Comment

Improved Cellometer Auto 2000 Cell Viability Counter for Primary Cell Analysis

Nexcelom is pleased to offer an even faster Auto 2000 Cell Viability Counter. Key features and capabilities of the Auto 2000 include: Determination of concentration and viability of primary cells in 30 seconds! Counting of live / dead nucleated cells in samples containing red blood cells Analysis of cells from peripheral blood, cord blood, and bone marrow without lysing User-friendly touch screen with many pre-set assays Cell images for visualization of samples and counted cells Auto-save option for archiving of images and concentration / viability data Pre-optimized assays include: PBMC Concentration & Viability Stem Cell Concentration & Viability Nucleated Cell Concentration [...]

By |2021-06-15T20:53:47+00:00March 28th, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments

ViaStain™ Viability Dyes

ViaStain™ Viability Dyes Nexcelom offers several dyes for fluorescence viability determination. AO (acridine orange) is a nuclear staining dye used to identify and count nucleated cells in a heterogeneous cell population. PI (propidium iodide) is a nuclear staining dye that only enters cells with compromised membranes. It is used to identify dead nucleated cells. AO/PI is a mixture of acridine orange and propidium iodide nuclear staining dyes. The dye mixture can be used with the Cellometer Auto 2000 and Cellometer Vision dual-fluorescence instruments to accurately count live and dead nucleated cells within heterogeneous samples containing non-nucleated cells, such as red [...]

By |2021-06-15T20:53:58+00:00March 28th, 2012|Categories: Cell Counting Leadership, Cellometer Product News|0 Comments
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