Blog | Page 25 of 26 | Nexcelom Bioscience

Cellometer PI Cell Cycle Kit

Nexcelom is pleased to announce the first cell-based assay kit specifically developed for use with the Cellometer Vision CBA Analysis System. The Cellometer PI Cell Cycle Kit (CSK0112) includes a pre-mixed staining solution sufficient for 100 tests and a comprehensive instruction booklet that covers sample preparation, imaging, and data analysis using Cellometer Vision CBA and FCS Express software. Product #: CSK0112 Description: Cellometer PI Cell Cycle Kit Size: 100 tests Key Kit Features: No Washing. No Clogging. – Image-based analysis; no fluidics Simple Set-up: No daily instrument validation or calibration User-Friendly Procedure: Simple fixing and staining protocol, pre-defined assay in the Vision CBA instrument with optimized [...]

By Polly Mitchell|2021-06-15T20:51:34+00:00June 21st, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments

Direct Comparison of Different Stem Cell Types and Subpopulations Reveals Superior Paracrine Potency and Myocardial Repair Efficacy with Cardiosphere-Derived Cells

Direct Comparison of Different Stem Cell Types and Subpopulations Reveals Superior Paracrine Potency and Myocardial Repair Efficacy With Cardiosphere-Derived Cells Authors: Tao-Sheng Li, PhD., Ke Cheng, PhD., Konstantinos Malliaras, MD, et al. Journal of the American College of Cardiology 2012; 59(10): 942-953; doi:10.1016/j.jacc.2011.11.029. Background: Many types of stem cells have been used clinically in attempts to treat damaged hearts. The discovery of cardiac stem cells within the heart and the development of a technique for expansion of cardiosphere-derived cells (CDCs) has given researchers a new option in the use of stem cells for regenerative therapy. Purpose: To evaluate the success [...]

By Polly Mitchell|2021-06-15T20:51:45+00:00June 21st, 2012|Categories: Publication Review, Stem Cell/Regenerative Medicine|0 Comments

AO/PI Viability

Increase Accuracy, Remove Lysing Steps, and Optimize Productivity with Dual-Fluorescence AO/PI Staining and Multi-Parameter Viability Data From Bone Marrow, Cord Blood, Peripheral Blood, Tissue, and Other Sample Types

By Polly Mitchell|2021-06-15T20:51:57+00:00June 14th, 2012|Categories: About Cell-Based Assays, Cell Counting Leadership|2 Comments

Jurkat Cells

Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available. Jurkat cells are widely used for IL-2 production and in the study of acute T cell leukemia, T cell signaling, and the effectiveness of potential anti-cancer compounds. Jurkat cells were used to establish the Nexcelom Apoptosis (Annexin V-FITC / PI) and PI Cell Cycle protocols for the Vision CBA [...]

By Polly Mitchell|2021-06-15T20:52:08+00:00June 13th, 2012|Categories: About Cells, Cell Counting Leadership|0 Comments

Measure total nucleated cells collected by Apheresis

Apheresis is the process of removing a specific component of the blood, such as platelets, red blood cells, plasma (liquid part of the blood) or granulocytes (white blood cells) and returning the remaining components to the donor. Leukapheresis is a specific kind of apheresis in which white blood cells are separated from a sample of blood. Cells contained in a leukapheresis product is mainly nucleated cells, some residual red blood cells and platelets. Application: measure live /dead nucleated cell concentration and viability Sample Type: human peripheral blood Leukapheresis Cell Type: total nucleated cells Staining Method: acridine orange and propidium iodide (Ao/PI) Instrument: [...]

By Jean Qiu|2021-06-15T20:52:19+00:00May 1st, 2012|Categories: Cell Counting Leadership, Cellometer Application News|0 Comments

Identification of Estrogen Receptor Dimer Selective Ligands Reveals Growth-Inhibitory Effects on Cells That Co-Express ER(alpha) and ER(beta)

Powell, E . et al. (2012) PLoS ONE. 7(2):e30993 doi:10.1371/journal.pone.0030993 -Trypan blue viability using Cellometer

By Polly Mitchell|2021-06-15T20:52:40+00:00April 27th, 2012|Categories: CAR T / Cell Therapy, Cellometer User Publications|0 Comments

Targeting Radioresistant Osteosarcoma Cells with Parthenolide

Zuch, D., et al. (2012) J. Cell. Biochem. 113: 1282-1291. -Analysis of cell growth using Cellometer

By Polly Mitchell|2021-06-15T20:52:47+00:00April 27th, 2012|Categories: Cellometer User Publications, Oncology / Tumor Microenvironment|0 Comments

Coordinated Regulation of Neutrophil Homeostasis by Liver X Receptors in Mice

Hong, C., et al. (2012). J. Clin Invest. 122(1): 337-347. -Trypan blue viability using Cellometer

By Polly Mitchell|2021-06-15T20:52:56+00:00April 27th, 2012|Categories: Animal Models, Cellometer User Publications|0 Comments

Trypan Blue Viability

Trypan Blue Viability: Viability is a measure of the metabolic state of a cell population which is indicative of the potential for growth. Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. Trypan blue dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging. Within 30 seconds, the Cellometer Auto T4 instrument counts both live cells and dead cells [...]

By Polly Mitchell|2021-06-15T20:53:08+00:00April 13th, 2012|Categories: About Cell-Based Assays, Cell Counting Leadership|0 Comments

Hepatocytes

Hepatocytes are the predominant cell in the liver, comprising about 60% of liver cells and 80% of liver mass. One of the main hepatocyte functions is the production of bile. Hepatocytes are also responsible for the regulation of metabolism, detoxification, and the manufacture of important circulating proteins, making them a critical component of studies involving liver disease, drug metabolism, and toxicology. […]

By Polly Mitchell|2021-06-15T20:53:16+00:00March 30th, 2012|Categories: About Cells, Cell Counting Leadership|0 Comments