Use Cellometer Vision to measure nucleated cell concentration in whole blood without lysing RBC

Cellometer Vision incorporates image based cell counting and fluorescence detection in a compact and easy-to-use instrument. With fluorescence detection capabilities, Cellometer Vision is an ideal solution for many complex cell population characterization assays such as rapidly counting white-blood-cells in whole blood. Acridine orange (AO) is a fluorescent nucleic acid stain that has been used to measure nucleated cell concentration in various cell types, including mammalian cell lines, mammalian primary cells, and yeasts. AO is a membrane-permeable dye that stains nucleated cells green.   The following article describes a simple and specific immunoassay to detect cellular immune [...]

By |2021-06-15T20:45:32+00:00March 29th, 2013|Categories: Cellometer, Cellometer Application News, Instrument|0 Comments

Quantification of Pathogenic Yeast / Fungi

Rapid Quantification of Pathogenic Fungi by Cellometer Image-Based Cytometry Joint collaboration between Nexcelom Bioscience and Merrimack College Objective: To demonstrate a simple image cytometry method for the quantification of viable pathogenic fungi, Histoplasma capsulatum. To validate image cytometry as a viable alternative method to CFU counting, a method that is time-consuming (sometimes taking 1-2 weeks for the formation of visible colonies), labor-intensive, and limited in efficiency and sensitivity. To validate image cytometry as a viable alternate to flow cytometry, due to the high cost, complexity, and biosafety containment issues associated with the use of flow cytometry for analysis of pathogenic [...]

1-Step Yeast Concentration and Yeast Viability: Fermentation Monitoring for Brewing

Monitoring of yeast viability during the fermentation process is an important quality control procedure employed by the brewing industry. Consistent yeast viability during the fermentation process is critical for batch-to-batch consistency and overall product quality. The Cellometer Auto X4 (10x) Fluorescent Cell Counter is specifically optimized for simple, 1-step determination of yeast concentration and viability and is ideal for both small and large breweries looking to automate their fermentation monitoring. Performance of the Cellometer Auto X4 (10x) has been proven in the largest breweries in the U.S. […]

Cellometer PI Cell Cycle Kit

Nexcelom is pleased to announce the first cell-based assay kit specifically developed for use with the Cellometer Vision CBA Analysis System. The Cellometer PI Cell Cycle Kit (CSK0112) includes a pre-mixed staining solution sufficient for 100 tests and a comprehensive instruction booklet that covers sample preparation, imaging, and data analysis using Cellometer Vision CBA and FCS Express software. Product #: CSK0112 Description: Cellometer PI Cell Cycle Kit Size: 100 tests Key Kit Features: No Washing. No Clogging. – Image-based analysis; no fluidics Simple Set-up: No daily instrument validation or calibration User-Friendly Procedure: Simple fixing and staining protocol, pre-defined assay in the Vision CBA instrument with optimized [...]

By |2021-06-15T20:51:34+00:00June 21st, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments

Improved Cellometer Auto 2000 Cell Viability Counter for Primary Cell Analysis

Nexcelom is pleased to offer an even faster Auto 2000 Cell Viability Counter. Key features and capabilities of the Auto 2000 include: Determination of concentration and viability of primary cells in 30 seconds! Counting of live / dead nucleated cells in samples containing red blood cells Analysis of cells from peripheral blood, cord blood, and bone marrow without lysing User-friendly touch screen with many pre-set assays Cell images for visualization of samples and counted cells Auto-save option for archiving of images and concentration / viability data Pre-optimized assays include: PBMC Concentration & Viability Stem Cell Concentration & Viability Nucleated Cell Concentration [...]

By |2021-06-15T20:53:47+00:00March 28th, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments
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