Instrument Archives | Page 22 of 26

Ignyta tests Celigo against Cell Titer-Glo for cell proliferation

Here's a great example of how the Celigo image cytometer is able to perform common experiments while saving time and money! Ignyta, Inc. was looking for a new way to perform reagent-free proliferation analyses with suspension cells. This new method had to produce results which correlated well to their current method, Cell Titer-Glo®. Nexcelom and Ignyta partnered to perform a head-to-head cell proliferation comparison between Celigo® and Cell Titer-Glo. Using four suspension cell types (Ba/F3 parental cell line, Ba/F3 expressing an oncogenic gene, oncogenic gene mutant A and B), Ignyta plated all cells at a concentration of 5,000 cells/well in [...]

By Polly Mitchell|2021-06-15T19:47:39+00:00October 23rd, 2015|Categories: Celigo, Celigo User Publications, Instrument|Tags: Celigo, Cell Proliferation, Image Cytometer|0 Comments

Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By Leo Chan|2021-06-15T19:47:54+00:00October 21st, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: B220, CD4, CD5, CD8, immunophenotyping, Surface marker, White Paper|0 Comments

UNM College of Pharmacy uses Cellometer to perform TB and AO/PI Viability

Here's a great example of how the Cellometer is being used by researchers in toxicology. The University of New Mexico College of Pharmacy investigated the mechanisms by which environmentally-relevant concentrations of inorganic arsenic contribute to genotoxicity. Inorganic arsenic is present in the air, food, and drinking water in many countries and has been linked to various cancers, skin lesions, and immune system disorders, although the exact mechanisms are not yet clear. To study arsenic’s genotoxic mechanisms, the researchers employed Nexcelom’s Cellometer to assess cell number and viability of primary mouse thymus cells and the murine thymus pre-T cell line, D1 [...]

By Polly Mitchell|2021-06-15T19:48:26+00:00October 19th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer, toxicology, Trypan Blue|0 Comments

Live Webinar! Join us for “3D Tumor Spheroids Based Functional Assays”

Recent publications have suggested that using 3D tumor spheroids is a more predictive model for preclinical research. Nexcelom Bioscience has developed a standardized microplate method for rapid generation, imaging and analysis of 3D tumor spheroids using the Celigo image cytometer. 40 cancer cell lines' ability to form spheroids, optimal seeding densities and culture conditions have been established. Protocols measuring tumor growth, viability, migration and invasion have been utilized by many researchers for routine preclinical drug studies. If you are interested to learn more about how your research might benefit from working with 3D tumor spheroids, this is a great place to start! [...]

By Polly Mitchell|2021-06-15T19:48:33+00:00October 13th, 2015|Categories: 3D Models, Celigo, Celigo Application News, Instrument|Tags: 3D Tumor Spheroids, Celigo, webinar|0 Comments

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:44+00:00October 7th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Aphidicolin, Cell Cycle, Etoposide, Nocodazole, White Paper|0 Comments

Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:56+00:00September 23rd, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Apoptosis, CCCP, Mitochondrial Potential, Necrosis, White Paper|0 Comments

Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer
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Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer

Bioprocessing/Cell Live Development, Celigo, Celigo Application News, Instrument

Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer

Celigo Imaging Cytometer demonstrated the utility of automation in the development and monitoring of new CHO-based cell lines for increasing efficiency in cell line development.

By Leo Chan|2021-06-15T19:49:18+00:00September 9th, 2015|Categories: Bioprocessing/Cell Live Development, Celigo, Celigo Application News, Instrument|Tags: Cell Line Development, White Paper|0 Comments

Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements [12]. Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]

By Leo Chan|2021-06-15T19:49:43+00:00August 19th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Stromal Vascular Fraction (SVF), Viability, White Paper|0 Comments

A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]

By Leo Chan|2021-06-15T19:49:50+00:00August 5th, 2015|Categories: Celigo, Celigo Application News, Instrument|Tags: Cell Proliferation, Drug toxicity, Label-free, White Paper|0 Comments

Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]

By Leo Chan|2021-06-15T19:50:01+00:00July 22nd, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: B Cells, B1, B2, Cell Proliferation, White Paper|0 Comments