Instrument Archives | Page 21 of 26

Celigo evaluates a plant extract for glioblastomoa multiforme treatment

At the Canary Center at Stanford for Early Cancer Detection, investigators studied how AshwaMAX (a steroidal lactone from a winter cherry plant, Withania somnifera, extract) might work as an oral treatment for those with the highly aggressive cancer glioblastoma multiforme (GBM). A heterogeneous disease, non-specific therapies for GBM have proven largely ineffective. Two patient-derived GBM lines (GBM2, GBM39) and one GBM cell line were cultured to create neurospheres that were then exposed to various concentrations of AshwaMAX. Celigo measured cell proliferation and cell death via Trypan Blue staining. AshwaMAX inhibited the neurospheres at nanomolar concentrations. After additional work in vivo, [...]

By Polly Mitchell|2021-06-15T19:44:29+00:00December 1st, 2015|Categories: Celigo, Celigo User Publications, Instrument|Tags: Cancer Cell Lines, Celigo, cell death, Cell Proliferation, Image Cytometer, image cytometry, Imaging Cytometer, Imaging Cytometry, Trypan Blue|0 Comments

Cellometer investigates new treatment paradigm for advanced endometrial cancer

University of North Carolina researchers investigated different techniques for inhibiting the catalytic activity of protein hTERT – a marker of advanced stage endometrial cancer. Endometrial cancer cell lines ECC-1 and Ishikawa were exposed to either siRNA or a small molecule pharmacological inhibitor BIBR1532, in addition to the drug paclitaxel, to see whether inhibiting hTERT provided additional efficacy against these cancer cells. The Cellometer, in combination with propidium iodide and Annexin-V FITC, calculated apoptosis in the various treatment conditions. The hTERT inhibition plus paclitaxel did prove synergistic, reducing cell growth and invasion more than paclitaxel alone. Furthermore, BIBR1532 antagonized cell invasion [...]

By Polly Mitchell|2021-06-15T19:44:51+00:00November 23rd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Annexin-V, Cancer Cell Lines, Cellometer, Image Cytometer, image cytometry, Imaging Cytometer, Imaging Cytometry, Propidium Iodide|0 Comments

Celigo Advances studies in T Cell Therapy as a pediatric CHOP patient defeats her recurrent leukemia with immunotherapy

Emily Whitehead, cancer survivor, holding Nexcelom cell counting sheep. Thanks to the lifesaving T cell therapy clinical trial at Children’s Hospital of Philadelphia, Emily Whitehead is now two years cancer free. Diagnosed with acute lymphoblastic leukemia (ALL) right after her 5th birthday, doctors discovered that Emily’s leukemia was particularly resistant to chemotherapy, as are roughly 15% of the total number of ALL cases. After two recurrences of the disease, Emily’s parents enrolled her in a clinical trial for CTL019, an experimental therapeutic using a patient’s own reprogrammed T cells to eliminate the cancer cells. Now 9 years old, [...]

By Polly Mitchell|2021-06-15T19:45:08+00:00November 20th, 2015|Categories: CAR T / Cell Therapy, Celigo, Celigo Application News, Instrument|Tags: Cancer Cell Lines, Celigo, Image Cytometer, image cytometry, leukemia, T cell therapy, T cells|0 Comments

Cellometer Used in study to Establish Baseline Values of Autism Biomarkers in Saliva

University of Minnesota scientists analyzed the saliva of twelve “neurotypical” adult subjects for the levels of sixteen autism-associated biomarkers (glutamine, glutamic acid, CD-26, C4B, IFN-γ, MT-2, testosterone, IL-12, Carnitine, GSH, GSSG, cystine, GABA, serotonin, cortisol and melatonin) in the hopes that establishing baseline levels of these molecules in a control population might help identify autism-associated changes in non-neurotypical subjects in the future. The Cellometer was used to establish accurate cell counts throughout the experiment. Ten biomarkers were successfully measured and the sample collection protocol proved to be non-stressful and easy enough to use on a broad range of subjects. You [...]

By Polly Mitchell|2021-06-15T19:45:57+00:00November 18th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autism, Cell Counting, Cellometer|0 Comments

Cellometer K2 aids in Anti-Cancer Immunity Research

The MD Anderson Cancer Center investigated how a patient’s anti-cancer T cells might be better protected from the cytotoxic effects of the anti-thymidylate drugs (AThys; such as methotrexate) used to treat lung, breast, colon, and pancreas cancers. Although AThys successfully attack cancer cells, the drugs also reduce a patient’s own anti-cancer T cell population, which plays an important role in helping the patient overcome the disease. Using Jurkat, AaPC cells, and mutated human proteins called muteins, researchers were able to manufacture T cells that were resistant to cytotoxic levels of AThys. The Cellometer K2 and Trypan Blue were used to [...]

By Polly Mitchell|2021-06-15T19:46:09+00:00November 16th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cancer Cell Lines, Cellometer K2, jurkat cells, T cells, Trypan Blue|0 Comments

Cellometer Vision provides new method for measuring cytotoxic potential in Natural Killer Cells

The MD Anderson Cancer Center worked in collaboration with Nexcelom to create a new method by which to measure the cytotoxic potential of natural killer (NK) cells. The traditional non-radioactive method, calcein release, is subject to variation, with differing dynamic ranges depending on tumor type. The Cellometer Vision and calcein, in combination with K562, 721.221, and Jurkat cells, were used to develop a novel, image cytometry-based assay to ascertain NK cytotoxicity. Using fluorescent intensity gating to ignore dimmer cells and apoptotic bodies, image cytometry provided a way to measure tumor cell lysis in a specific manner with the same experimental [...]

By Polly Mitchell|2021-06-15T19:46:24+00:00November 12th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer Vision, Image Cytometer, image cytometry, jurkat cells, natural killer cells|0 Comments

Researchers in Turkey use Cellometer for Trypan Blue Viability

Here’s another great example of how the Cellometer instruments are used to test viability of cells prior to plating. Standard therapy zoledronic acid (ZA) is typically used for metastatic bone disease in breast cancer patients, but it’s mechanism of action is not clear. Here, Turkish scientists investigated zoledronic acid with and without serine-threonine inhibitors in human MCF-7 and MDA-MB-231 breast cancer cells. Cellometer was used in combination with tryan blue to assess cell viability prior to plating. After plating, the cells were treated with various combinations of therapeutic agents. ZA with either one of the serine-threonine inhibitors examined proved more [...]

By Polly Mitchell|2021-06-15T19:46:40+00:00November 10th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Breast Cancer Drugs, Cellometer, Trypan Blue|0 Comments

French Institute for Health and Medical Research and the University of Lyon use Cellometer Auto T4 for Trypan Blue Viability

Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays: Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The [...]

By Polly Mitchell|2021-06-15T19:47:10+00:00November 6th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Rowan Univ Researchers using Cellometer Vision for PI Viability, Caspase-3 activation and ROS

The Cellometer instruments have a wide range of functionality – this publication demonstrates how an instrument such as the Cellometer Vision can be used for more than just viability and can perform many cell-based assays. Fructose differs from glucose in that it is not regulated by a negative feedback loop and can thereby negatively impact intracellular energy stores. As the incidence of diabetes and obesity have risen in this country, so has the intake of fructose, making fructose’s effects on pancreatic cells important to understand. Here, researchers at Rowan University revealed that fructose sensitizes pancreatic beta cells to TNF-a-induced toxicity. [...]

By Polly Mitchell|2021-06-15T19:47:19+00:00November 4th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, cell-based assays, Cellometer Vision|0 Comments

96- and 384-well Ultra-Low Adhesion Round-Bottom Multi-Well Plates now Available

384-well Ultra-low attachment treatedround bottom multi-well plates 96-well Ultra-low attachment treated round bottom multi-well plates Nexcelom has just released two new products: Ultra-low adhesion round-bottom plates in 96-well and 384-well formats that are specifically designed for working with 3D tumor spheroids. These clear wall, clear bottom plates were validated with U87 MG cells and tracked using a bright-field imaging technique on the Celigo Image Cytometer. These plates are ideal for single spheroid drug screening assays and can also be used to investigate spheroid invasion with the addition of Matrigel. You can read more about the plates [...]

By Polly Mitchell|2021-06-15T19:47:30+00:00October 27th, 2015|Categories: Cellometer, Cellometer Product News, Instrument|Tags: 384-well plates, 3D Tumor Spheroids, 96-well plates, Celigo, drug screening|0 Comments