Instrument Archives | Page 19 of 26

Cellometer helps to study microalgae metabolism for future industrial biotechnology applications

Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.

By Polly Mitchell|2021-06-15T19:13:45+00:00February 16th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cell Counting, Cellometer, lipids, microalgae, starch|0 Comments

Celigo assists in optimizing CHO cells for biopharmaceutical production

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]

By Polly Mitchell|2021-06-15T19:13:58+00:00February 11th, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: 96-well plates, Celigo, Cell Counting, CHO, CHO cells, Hoechst, Novo Nordisk, Propidium Iodide|0 Comments

Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

By Polly Mitchell|2021-06-15T19:14:19+00:00February 9th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: aspirin, Cancer Cell Lines, Cell Cycle, Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells

Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]

By Polly Mitchell|2021-06-15T19:14:39+00:00February 4th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: AO/PI, Cell Counting, Cellometer Auto 2000, UCMSCs, umbilical cord cells|0 Comments

BrewDog embraces and endorses Cellometer X2 technology as they expand

In 2007 two guys (and their dog) set out on a journey to revolutionize the brewing industry. With a passion for flavor, quality and a love of craft brewing, they created the BrewDog brand. Almost a decade later they are by far one of the world’s premier craft brewers, with an ever-expanding British brewery, 26 independent bars around the world, and range of brews that have become the staple of the craft connoisseurs. Along with a huge export business comes the breaking of ground on BrewDog’s new US brewery (Ohio). In order to continue producing the highest quality beers consistently [...]

By Polly Mitchell|2021-06-15T19:14:56+00:00February 2nd, 2016|Categories: Brewery / Bakery / Biofuel, Cellometer, Cellometer Product News, Instrument|Tags: BrewDog, Brewing, Cellometer X2, craft beer, Yeast, yeast viability|0 Comments

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

By Polly Mitchell|2021-06-15T19:15:14+00:00January 28th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: Apoptosis, Cell Cycle, human leukemia, lead paint, Propidium Iodide|0 Comments

Celigo evaluates quinomycin A as a possible therapeutic tool for pancreatic cancer

The Notch-disrupting and cancer stem cell-inhibiting effects of the drug quinomycin A were investigated at the University of Kansas Medical Center. Using human pancreatic cancer cells PanC-1, MiaPaCa-2, and BxPC-3 and the Celigo to determine the number and size of pancreatospheres, researchers evaluated the drug’s ability to block cancer stem cell growth via inhibition of the Notch signaling pathway. After administration of the drug, proliferation and colony formation were blocked in cancer cell lines but not in normal pancreatic epithelial cells. Furthermore, cancer stem cell markers were reduced as was pancreatosphere formation. This work identifies quinomycin A as an efficacious [...]

By Polly Mitchell|2021-06-15T19:15:28+00:00January 26th, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: Cancer Cell Lines, Celigo|0 Comments

Celigo furthers studies of homologous recombination DNA repair machinery

The Danish Cancer Society Research Center recently published a study furthering their analysis of homologous recombination DNA repair machinery. The group previously reported on a growth factor, PSIP1, that enables DNA end resection. With GFP-transfected U2OS cells, the group investigated a structurally similar protein, hepatoma-derived growth factor-related protein 2 (HDGFRP2). The Celigo analyzed cell number and viability via fluorescent markers. The group reports that HDGRFP2 may help to repair silent genes that have been impaired or active genes inhibited by DNA damage. Read the full publication here.

By Polly Mitchell|2021-06-15T19:15:44+00:00January 21st, 2016|Categories: Celigo, Celigo User Publications, Instrument|Tags: Celigo, Image Cytometer, image cytometry|0 Comments

Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale

Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]

By Polly Mitchell|2021-06-15T19:16:05+00:00January 19th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: Cell Counting, Cell Viability, Cellometer Auto 2000, hMSC, regenerative medicine|0 Comments

Cellometer helps with culturing optimization necessary for future cell-based therapies

EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]

By Polly Mitchell|2021-06-15T19:16:26+00:00January 14th, 2016|Categories: CAR T / Cell Therapy, Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, cell-based therapy, Cellometer, mesenchymal stem cells, Stem Cell|0 Comments