NK92 cell-mediated ADCC using calcein AM
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231
Kinetically monitor multicellular tumor spheroid (MCTS) growth & Propidium Iodide (PI) dead cell signal on U87MG Glioblastoma over mulitple days
Measure the effects of a panel of drugs on the viability of U87MG Glioblastoma MCTS using calcein AM and Propidium Iodide fluorescent staining
Measure NK cell-mediated cytotoxicity using calcein AM-stained K562 and IMR32 cells
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations
To analyze transfection efficiency over a 4 day period when treated with various amounts of a transfection compound.
In this experiment, we demonstrate the capability of Celigo to measure cell count on microcarrier beads and infected cells positively staining for AlexaFluor488-labeled antibody again the viral protein.
To measure the level of receptor internalization induced by known antibody
Celigo will be able to perform rapid, whole-well imaging and analysis of Ki-67 expression levels to compare between groups.
By measuring the number of Caspase 3/7 positive cells, we can determine the counts of apoptotic cells in the population