Cellometer Archives | Page 9 of 13

Cellometer assists in search to combat tumor radio-resistance

At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]

By Polly Mitchell|2021-06-15T19:18:34+00:00December 29th, 2015|Categories: Cellometer, Cellometer Application News, Instrument, Oncology / Tumor Microenvironment|Tags: Cell Counting, Cellometer, Trypan Blue|0 Comments

Cellometer used in study to examine secondary effects of MAO inhibitors

In the “catecholaldehyde hypothesis” of Parkinson’s disease neurodegeneration, cytoplasmic dopamine (DA) is converted to 3,4-dihydroxyphenylacetaldehyde (DOPAL) by the enzyme monoamine oxidase-A (MAO-A). DOPAL is a toxic substance, generating free radicals and inhibiting mitochondrial function. Scientists at NIH wanted to compare the abilities of currently available MAO-A and MAO-B inhibiting drugs at decreasing endogenous DOPAL levels. Using PC12 cells, six drugs were tested for their effects on DOPAL: MAO-A inhibitor clorygyline, three reversible MAO-A inhibitors, and the MAO-B inhibitors selegiline and rasagiline. The Cellometer was used to count the cells throughout experimentation. Clorgyline, rasagiline, and selegiline decreased DOPAL levels in both [...]

By Polly Mitchell|2021-06-15T19:43:09+00:00December 22nd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer, MAO inhibitors, NIH, Parkinson's|0 Comments

Cellometer assists in characterization of adipocytic extracellular vesticles

Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]

By Polly Mitchell|2021-06-15T19:43:21+00:00December 17th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: adipocytes, Cell Counting, Cellometer, obesity, Trypan Blue|0 Comments

Cellometer participates in study to ascertain rotenone mechanism of action

A common hypothesis concerning the pathogenesis of Parkinson’s neurodegeneration suggests that cytoplasmic dopamine (DA) is converted to the auto-toxic 3,4-dihydroxyphenylacetaldehyde (DOPAL), which is neutralized by aldehyde dehydrogenase (ALDH). NIH researchers studied whether rotenone, a substance typically used to create in vitro models of Parkinson neurodegeneration, inhibits ALDH function. PC12 cells were exposed to various concentrations of rotenone in the presence or absence of F-dopamine. Catecholamine metabolism was then measured. The Cellometer maintained cell counts throughout experimentation. Rotenone increased DOPAL while decreasing dopamine levels in cell culture, but in a test tube, the DOPAL to DOPAC conversion by ALDH was not [...]

By Polly Mitchell|2021-06-15T19:43:33+00:00December 15th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer, NIH, Parkinson's|0 Comments

Cellometer helps to elucidate underlying mechanism of hypomyelination

Vanderbilt University researchers sought to evaluate the role of mTor signaling during myelination. Other work suggests that myelin abnormalities may be distinct from cortical lesions, and these scientists investigated the underlying mechanisms of hypomyelination to support or refute the other data. Primary astrocytes from knock out mouse pups were isolated to ascertain whether the astroglyosis seen in these mouse models is a specific or non-specific consequence of altered oligodendrocyte structure. Cellometer analyzed spherical size of these cells during experimentation. Although the findings suggest it is a nonspecific response, more study is clearly needed. These findings also suggest a dissociation between [...]

By Polly Mitchell|2021-06-15T19:43:40+00:00December 10th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: cell size, Cellometer|0 Comments

Cellometer X4 aides in assessing the toxic effects of silver nanoparticles

East Carolina University set out to elucidate the possible cardiovascular impacts that may occur after exposure to silver nanoparticles (AgNP), which are currently being assessed for use in a broad range of biomedical and consumer applications including wound dressings, laundry detergents, deodorants, and clothing. Despite their seeming utility, their downstream toxicity, especially in relation to the heart upon inhalation of aerosolized particles, has not been fully studied. Sprague Dawley rats were exposed to AgNP intratracheally for 7 days before branchoalveolar lavage was performed. The Cellometer Auto X4 was used on the contents of the resulting cell pellet to ascertain total [...]

By Polly Mitchell|2021-06-15T19:43:48+00:00December 8th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer Auto X4, total cell count|0 Comments

Cellometer Auto T4 is used to uncover novel targets for Graves’ Orbitopathy therapeutics

Researchers at Cardiff University and First Affiliated Hospital of the Fourth Military Medical University of Shaanxi, China investigated the mechanisms by which excessive adipogenesis and extracellular matrix production, namely in the form of hyaluronan acid (HA), expand orbital contents in Graves’ Orbitopathy (GO) patients. Currently, GO patients are treated with immunosuppressive and anti-inflammatory therapies that are largely ineffective. This study examined FOXOs and how their involvement in the PI3K pathway might be studied in order to uncover non-immunosuppressive therapy targets. Preadipocytes/fibroblasts (PFs) were used to study the outcomes when FOXO expression was enhanced and inhibited. The Cellometer Auto T4 and [...]

By Polly Mitchell|2023-05-04T15:39:16+00:00December 3rd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Cellometer investigates new treatment paradigm for advanced endometrial cancer

University of North Carolina researchers investigated different techniques for inhibiting the catalytic activity of protein hTERT – a marker of advanced stage endometrial cancer. Endometrial cancer cell lines ECC-1 and Ishikawa were exposed to either siRNA or a small molecule pharmacological inhibitor BIBR1532, in addition to the drug paclitaxel, to see whether inhibiting hTERT provided additional efficacy against these cancer cells. The Cellometer, in combination with propidium iodide and Annexin-V FITC, calculated apoptosis in the various treatment conditions. The hTERT inhibition plus paclitaxel did prove synergistic, reducing cell growth and invasion more than paclitaxel alone. Furthermore, BIBR1532 antagonized cell invasion [...]

By Polly Mitchell|2021-06-15T19:44:51+00:00November 23rd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Annexin-V, Cancer Cell Lines, Cellometer, Image Cytometer, image cytometry, Imaging Cytometer, Imaging Cytometry, Propidium Iodide|0 Comments

Cellometer Used in study to Establish Baseline Values of Autism Biomarkers in Saliva

University of Minnesota scientists analyzed the saliva of twelve “neurotypical” adult subjects for the levels of sixteen autism-associated biomarkers (glutamine, glutamic acid, CD-26, C4B, IFN-γ, MT-2, testosterone, IL-12, Carnitine, GSH, GSSG, cystine, GABA, serotonin, cortisol and melatonin) in the hopes that establishing baseline levels of these molecules in a control population might help identify autism-associated changes in non-neurotypical subjects in the future. The Cellometer was used to establish accurate cell counts throughout the experiment. Ten biomarkers were successfully measured and the sample collection protocol proved to be non-stressful and easy enough to use on a broad range of subjects. You [...]

By Polly Mitchell|2021-06-15T19:45:57+00:00November 18th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autism, Cell Counting, Cellometer|0 Comments

Cellometer K2 aids in Anti-Cancer Immunity Research

The MD Anderson Cancer Center investigated how a patient’s anti-cancer T cells might be better protected from the cytotoxic effects of the anti-thymidylate drugs (AThys; such as methotrexate) used to treat lung, breast, colon, and pancreas cancers. Although AThys successfully attack cancer cells, the drugs also reduce a patient’s own anti-cancer T cell population, which plays an important role in helping the patient overcome the disease. Using Jurkat, AaPC cells, and mutated human proteins called muteins, researchers were able to manufacture T cells that were resistant to cytotoxic levels of AThys. The Cellometer K2 and Trypan Blue were used to [...]

By Polly Mitchell|2021-06-15T19:46:09+00:00November 16th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cancer Cell Lines, Cellometer K2, jurkat cells, T cells, Trypan Blue|0 Comments