Cellometer Archives | Page 8 of 13

Cellometer helps to study microalgae metabolism for future industrial biotechnology applications

Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.

By Polly Mitchell|2021-06-15T19:13:45+00:00February 16th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cell Counting, Cellometer, lipids, microalgae, starch|0 Comments

Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

By Polly Mitchell|2021-06-15T19:14:19+00:00February 9th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: aspirin, Cancer Cell Lines, Cell Cycle, Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells

Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]

By Polly Mitchell|2021-06-15T19:14:39+00:00February 4th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: AO/PI, Cell Counting, Cellometer Auto 2000, UCMSCs, umbilical cord cells|0 Comments

BrewDog embraces and endorses Cellometer X2 technology as they expand

In 2007 two guys (and their dog) set out on a journey to revolutionize the brewing industry. With a passion for flavor, quality and a love of craft brewing, they created the BrewDog brand. Almost a decade later they are by far one of the world’s premier craft brewers, with an ever-expanding British brewery, 26 independent bars around the world, and range of brews that have become the staple of the craft connoisseurs. Along with a huge export business comes the breaking of ground on BrewDog’s new US brewery (Ohio). In order to continue producing the highest quality beers consistently [...]

By Polly Mitchell|2021-06-15T19:14:56+00:00February 2nd, 2016|Categories: Brewery / Bakery / Biofuel, Cellometer, Cellometer Product News, Instrument|Tags: BrewDog, Brewing, Cellometer X2, craft beer, Yeast, yeast viability|0 Comments

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

By Polly Mitchell|2021-06-15T19:15:14+00:00January 28th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: Apoptosis, Cell Cycle, human leukemia, lead paint, Propidium Iodide|0 Comments

Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale

Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]

By Polly Mitchell|2021-06-15T19:16:05+00:00January 19th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Stem Cell/Regenerative Medicine|Tags: Cell Counting, Cell Viability, Cellometer Auto 2000, hMSC, regenerative medicine|0 Comments

Cellometer helps with culturing optimization necessary for future cell-based therapies

EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]

By Polly Mitchell|2021-06-15T19:16:26+00:00January 14th, 2016|Categories: CAR T / Cell Therapy, Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, cell-based therapy, Cellometer, mesenchymal stem cells, Stem Cell|0 Comments

Cellometer T4 studies immune response to Delftia bacteria sometimes found in IV catheters

North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]

By Polly Mitchell|2021-06-15T19:17:14+00:00January 12th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cell Viability, Cellometer Auto T4, immune system, Trypan Blue|0 Comments

Cellometer T4 work in Adoptive Cell Transfer examines gender differences in pathology after stroke

Oregon Health & Science University researchers continued their studies into the gender-related differences in peripheral immune system response after stroke that produce greater downstream damage in males than females, and what role the spleen may play in those differences. The goal of this work was to uncover which subset of immune system cells may contribute to those pathogenic effects after a stroke and how they vary by gender. Using primary leukocytes from transgenic mice and adoptive T-cell transfer, scientists injected specific cell types into splenectomized male and female mice 24 hours after stroke was induced. The Cellometer Auto T4 maintained [...]

By Polly Mitchell|2021-06-15T19:17:51+00:00January 7th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, Cellometer Auto T4, immune system, stroke|0 Comments

Cellometer Auto T4 evaluates the cytotoxic potential of mouth rinses

How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]

By Polly Mitchell|2021-06-15T19:18:14+00:00January 5th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Counting, cell size, Cell Viability, Cellometer Auto T4, gingiva fibroblasts, mouthwash, Viability|0 Comments