Cellometer Archives | Page 13 of 13

Perform quick & easy cell based assays using Cellometer Image Cytometer

Using Cellometer Image Cytometer to Perform Consistent and Accurate Cell Cycle, Apoptosis, and GFP Analysis Cellometer K2 and Vision instruments are capable of not only accurately and quickly measuring the cell concentration and viability, but also have the capability to perform multiple cell-based assays. Below we highlight three such assays: Cell Cycle Apoptosis GFP Performing Cell Cycle Assay Analyze and compare the effects of different cell cycle pharmacological agents. Provides researchers with the ability to visually review and confirm counted cells. Use the provided FCS Express™ templates for quick and easy data analysis and presentation. Data acquired on the Cellometer [...]

By Dmitry Kuksin|2021-06-15T20:41:30+00:00April 24th, 2014|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Apoptosis, Cell Cycle, GFP, Image Cytometer|0 Comments

Get Flow Results without Flow Cytometry

Cellometer Vision CBA Image Cytometry System Incorporation of FCS Express 4 Software (De Novo Software) into the Vision CBA Analysis System has enabled users to conduct image-based analysis of cell-based assays requiring enhanced gating for the most accurate, reliable, and reproducible results. Side-by-side comparison of results for apoptosis, cell cycle, mitochondrial potential, autophagy, ABC transporter assays, aggresome detection, CD4+ / CD8+ immunophenotyping, and other assays has shown Cellometer image-based analysis results to be comparable to flow cytometry. Flow core labs can utilize the Vision CBA instrument to pre-screen samples prior to sorting and flow cytometry analysis and to [...]

By Polly Mitchell|2021-06-15T20:41:54+00:00March 24th, 2014|Categories: Cell Counting Leadership, Cellometer, Cellometer Product News, Instrument|Tags: Apoptosis, Cell Cycle, Cellometer Vision CBA, Flow Cytometry, GFP|0 Comments

Ten Advantages of Cellometer Automated Cell Counting

1. Streamline the QC Process Measure cell concentration and viability for multiple sample types using one method! No need to perform RBCs lysis for Clinical Samples: Bone Marrow, Cord Blood, Peripheral Blood, Mobilized Peripheral Blood, Tissue Digests Samples following cryopreservation Samples following density gradient separation Samples following magnetic separation (CD56+, CD34+) Cultured cells 2. Improve Accuracy of QC Results Dual-fluorescence counting eliminates interference from red blood cells, platelets, and debris 3. Reduce Testing Time Automated counting is 10x faster than manual counting 4. Reduce Errors Automated counting eliminates manual counting, calculation, and recording errors 5. Reduce Inter-Operator Variability Pre-set analysis parameters [...]

By Polly Mitchell|2021-06-15T20:42:11+00:00March 12th, 2014|Categories: Cell Counting Leadership, Cellometer, Cellometer Product News, Instrument|Tags: Cell Counting|0 Comments

Video Presentation: Using Cellometer Image Cytometry to Detect and Measure GFP Expression Efficiency

This presentation will focus on using Cellometer image cytometry to effectively determine GFP or other fluorescent protein transduction/transfection efficiency and cell concentration, as well as detect and quantify dual expression in a cell population. Quantifying GFP can be done in four easy steps. First pipette 20 ul of target cell sample into a disposable counting chamber. Then insert the slide into the instrument, select your assay from the drop-down menu, focus and click count. Within seconds the instrument acquires images, identifies cells with and without fluorescence and automatically tabulates the results. The results include the number of cells counted in [...]

By Dmitry Kuksin|2023-04-27T20:16:51+00:00January 27th, 2014|Categories: Cellometer, Cellometer Application News, Instrument|Tags: GFP, image cytometry, Video|0 Comments

Nexcelom has performed Cellometer image cytometry on all NCI-60 cancer cell lines

Nexcelom has performed Cellometer image cytometry analysis on all NCI-60 cancer cell lines.

By Dmitry Kuksin|2021-06-15T20:43:41+00:00January 13th, 2014|Categories: Cellometer, Cellometer Application News, Instrument, Oncology / Tumor Microenvironment|Tags: Cancer Cell Lines, image cytometry, NCI-60|0 Comments

Use Cellometer Vision to measure nucleated cell concentration in whole blood without lysing RBC

Cellometer Vision incorporates image based cell counting and fluorescence detection in a compact and easy-to-use instrument. With fluorescence detection capabilities, Cellometer Vision is an ideal solution for many complex cell population characterization assays such as rapidly counting white-blood-cells in whole blood. Acridine orange (AO) is a fluorescent nucleic acid stain that has been used to measure nucleated cell concentration in various cell types, including mammalian cell lines, mammalian primary cells, and yeasts. AO is a membrane-permeable dye that stains nucleated cells green. The following article describes a simple and specific immunoassay to detect cellular immune [...]

By Jean Qiu|2021-06-15T20:45:32+00:00March 29th, 2013|Categories: Cellometer, Cellometer Application News, Instrument|0 Comments

Quantification of Pathogenic Yeast / Fungi

Rapid Quantification of Pathogenic Fungi by Cellometer Image-Based Cytometry Joint collaboration between Nexcelom Bioscience and Merrimack College Objective: To demonstrate a simple image cytometry method for the quantification of viable pathogenic fungi, Histoplasma capsulatum. To validate image cytometry as a viable alternative method to CFU counting, a method that is time-consuming (sometimes taking 1-2 weeks for the formation of visible colonies), labor-intensive, and limited in efficiency and sensitivity. To validate image cytometry as a viable alternate to flow cytometry, due to the high cost, complexity, and biosafety containment issues associated with the use of flow cytometry for analysis of pathogenic [...]

By Polly Mitchell|2021-06-15T20:46:05+00:00November 9th, 2012|Categories: Brewery / Bakery / Biofuel, Cellometer, Cellometer Application News, Instrument|0 Comments

1-Step Yeast Concentration and Yeast Viability: Fermentation Monitoring for Brewing

Monitoring of yeast viability during the fermentation process is an important quality control procedure employed by the brewing industry. Consistent yeast viability during the fermentation process is critical for batch-to-batch consistency and overall product quality. The Cellometer Auto X4 (10x) Fluorescent Cell Counter is specifically optimized for simple, 1-step determination of yeast concentration and viability and is ideal for both small and large breweries looking to automate their fermentation monitoring. Performance of the Cellometer Auto X4 (10x) has been proven in the largest breweries in the U.S. […]

By Polly Mitchell|2021-06-15T20:47:07+00:00September 14th, 2012|Categories: Brewery / Bakery / Biofuel, Cellometer, Cellometer Application News, Instrument|0 Comments

Cellometer PI Cell Cycle Kit

Nexcelom is pleased to announce the first cell-based assay kit specifically developed for use with the Cellometer Vision CBA Analysis System. The Cellometer PI Cell Cycle Kit (CSK0112) includes a pre-mixed staining solution sufficient for 100 tests and a comprehensive instruction booklet that covers sample preparation, imaging, and data analysis using Cellometer Vision CBA and FCS Express software. Product #: CSK0112 Description: Cellometer PI Cell Cycle Kit Size: 100 tests Key Kit Features: No Washing. No Clogging. – Image-based analysis; no fluidics Simple Set-up: No daily instrument validation or calibration User-Friendly Procedure: Simple fixing and staining protocol, pre-defined assay in the Vision CBA instrument with optimized [...]

By Polly Mitchell|2021-06-15T20:51:34+00:00June 21st, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments

Improved Cellometer Auto 2000 Cell Viability Counter for Primary Cell Analysis

Nexcelom is pleased to offer an even faster Auto 2000 Cell Viability Counter. Key features and capabilities of the Auto 2000 include: Determination of concentration and viability of primary cells in 30 seconds! Counting of live / dead nucleated cells in samples containing red blood cells Analysis of cells from peripheral blood, cord blood, and bone marrow without lysing User-friendly touch screen with many pre-set assays Cell images for visualization of samples and counted cells Auto-save option for archiving of images and concentration / viability data Pre-optimized assays include: PBMC Concentration & Viability Stem Cell Concentration & Viability Nucleated Cell Concentration [...]

By Polly Mitchell|2021-06-15T20:53:47+00:00March 28th, 2012|Categories: Cellometer, Cellometer Product News, Instrument|0 Comments