Cellometer Archives | Page 10 of 13

Cellometer Vision provides new method for measuring cytotoxic potential in Natural Killer Cells

The MD Anderson Cancer Center worked in collaboration with Nexcelom to create a new method by which to measure the cytotoxic potential of natural killer (NK) cells. The traditional non-radioactive method, calcein release, is subject to variation, with differing dynamic ranges depending on tumor type. The Cellometer Vision and calcein, in combination with K562, 721.221, and Jurkat cells, were used to develop a novel, image cytometry-based assay to ascertain NK cytotoxicity. Using fluorescent intensity gating to ignore dimmer cells and apoptotic bodies, image cytometry provided a way to measure tumor cell lysis in a specific manner with the same experimental [...]

By Polly Mitchell|2021-06-15T19:46:24+00:00November 12th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer Vision, Image Cytometer, image cytometry, jurkat cells, natural killer cells|0 Comments

Researchers in Turkey use Cellometer for Trypan Blue Viability

Here’s another great example of how the Cellometer instruments are used to test viability of cells prior to plating. Standard therapy zoledronic acid (ZA) is typically used for metastatic bone disease in breast cancer patients, but it’s mechanism of action is not clear. Here, Turkish scientists investigated zoledronic acid with and without serine-threonine inhibitors in human MCF-7 and MDA-MB-231 breast cancer cells. Cellometer was used in combination with tryan blue to assess cell viability prior to plating. After plating, the cells were treated with various combinations of therapeutic agents. ZA with either one of the serine-threonine inhibitors examined proved more [...]

By Polly Mitchell|2021-06-15T19:46:40+00:00November 10th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Breast Cancer Drugs, Cellometer, Trypan Blue|0 Comments

French Institute for Health and Medical Research and the University of Lyon use Cellometer Auto T4 for Trypan Blue Viability

Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays: Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The [...]

By Polly Mitchell|2021-06-15T19:47:10+00:00November 6th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Rowan Univ Researchers using Cellometer Vision for PI Viability, Caspase-3 activation and ROS

The Cellometer instruments have a wide range of functionality – this publication demonstrates how an instrument such as the Cellometer Vision can be used for more than just viability and can perform many cell-based assays. Fructose differs from glucose in that it is not regulated by a negative feedback loop and can thereby negatively impact intracellular energy stores. As the incidence of diabetes and obesity have risen in this country, so has the intake of fructose, making fructose’s effects on pancreatic cells important to understand. Here, researchers at Rowan University revealed that fructose sensitizes pancreatic beta cells to TNF-a-induced toxicity. [...]

By Polly Mitchell|2021-06-15T19:47:19+00:00November 4th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, cell-based assays, Cellometer Vision|0 Comments

96- and 384-well Ultra-Low Adhesion Round-Bottom Multi-Well Plates now Available

384-well Ultra-low attachment treatedround bottom multi-well plates 96-well Ultra-low attachment treated round bottom multi-well plates Nexcelom has just released two new products: Ultra-low adhesion round-bottom plates in 96-well and 384-well formats that are specifically designed for working with 3D tumor spheroids. These clear wall, clear bottom plates were validated with U87 MG cells and tracked using a bright-field imaging technique on the Celigo Image Cytometer. These plates are ideal for single spheroid drug screening assays and can also be used to investigate spheroid invasion with the addition of Matrigel. You can read more about the plates [...]

By Polly Mitchell|2021-06-15T19:47:30+00:00October 27th, 2015|Categories: Cellometer, Cellometer Product News, Instrument|Tags: 384-well plates, 3D Tumor Spheroids, 96-well plates, Celigo, drug screening|0 Comments

Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By Leo Chan|2021-06-15T19:47:54+00:00October 21st, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: B220, CD4, CD5, CD8, immunophenotyping, Surface marker, White Paper|0 Comments

UNM College of Pharmacy uses Cellometer to perform TB and AO/PI Viability

Here's a great example of how the Cellometer is being used by researchers in toxicology. The University of New Mexico College of Pharmacy investigated the mechanisms by which environmentally-relevant concentrations of inorganic arsenic contribute to genotoxicity. Inorganic arsenic is present in the air, food, and drinking water in many countries and has been linked to various cancers, skin lesions, and immune system disorders, although the exact mechanisms are not yet clear. To study arsenic’s genotoxic mechanisms, the researchers employed Nexcelom’s Cellometer to assess cell number and viability of primary mouse thymus cells and the murine thymus pre-T cell line, D1 [...]

By Polly Mitchell|2021-06-15T19:48:26+00:00October 19th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, Cellometer, toxicology, Trypan Blue|0 Comments

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:44+00:00October 7th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Aphidicolin, Cell Cycle, Etoposide, Nocodazole, White Paper|0 Comments

Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:56+00:00September 23rd, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Apoptosis, CCCP, Mitochondrial Potential, Necrosis, White Paper|0 Comments

Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements [12]. Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]

By Leo Chan|2021-06-15T19:49:43+00:00August 19th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Stromal Vascular Fraction (SVF), Viability, White Paper|0 Comments