Use Celigo for ADCC Assay by Direct Cell Counting
Video: Perform qualitative and quantitative analysis for ADCC, CDC, and cell-mediated cytotoxicity assays using a single platform.
Use Celigo for 3D Tumor Spheroid-Based Functional Assays – Invasion into Matrigel
Video: 3D tumor spheroid invasion into Matrigel using u-bottom 96-well ULA plates. Examine drug effects on tumor spheroids as a model system for tumor invasion.
Introduction to the Celigo Image Cytometer
Celigo is a multi-channel imaging cell cytometer designed for scientists performing bench-top experiments in micro-well plates.
2D Cell Migration Assay using 96-well Oris™ Platypus Plate
Validate wound healing migration assay with OrisTM Platypus plate on Celigo
Transwell migration using DAPI
Image and count the number of cells which have migrated through the membrane of a transwell insert using fluorescent detection of DAPI-stained cells.
PBMC-mediated cytotoxicity using calcein AM
Measure PBMC-mediated cytotoxicity by counting total live K562 cells over time in the presence and absence of IL2
NK92 cell-mediated ADCC using calcein AM
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231
3D Multicellular Tumor Spheroid (MCTS) Kinetic Propidium Iodide Assay
Kinetically monitor multicellular tumor spheroid (MCTS) growth & Propidium Iodide (PI) dead cell signal on U87MG Glioblastoma over mulitple days
3D multicellular tumor spheroid (MCTS) endpoint viability screening assay
Measure the effects of a panel of drugs on the viability of U87MG Glioblastoma MCTS using calcein AM and Propidium Iodide fluorescent staining
NK cell-mediated cytotoxicity using calcein AM
Measure NK cell-mediated cytotoxicity using calcein AM-stained K562 and IMR32 cells