Leukocyte counts of BAL fluid BAL samples were mixed gently prior to diluting 50 ml BAL fluid with 50 ml of trypan blue (1:5 in 1xPBS) for viable cell counts. 20 ml of diluted cell sample were pipetted on a Cellometer counting chamber. Leukocytes were automatically counted using a Cellometer Auto T4 (Nexcelom Bioscience, Lawrence, MA, USA) […]
Viable T cells were enumerated every 7 days by trypan blue exclusion using the AutoT4 cell counter Cellometer (Nexcelom Bioscience, Lawrence, MA) […]
Canine adipose-derived mesenchymal stem cells (cASCs) When the monolayer of adherent cells reached approximately 70–80% confluence; trypsinization for cell splitting was performed using trypsin-EDTA solution (0.05%/0.02%, Biochrom AG, Berlin, Germany). After centrifugation, the cell pellets were subcultured at a concentration of 3 × 105cells/mL medium on 25cm2 tissue culture flasks containing DMEM/10% FCS. Cell counting was carried out by an automatic cell counter (Cellometer Nexcelom Bioscience, Lawrence, USA). The viable cell percentage from the harvested cells was calculated after trypan blue staining by an automatic cell counter (Cellometer Nexcelom Bioscience, Lawrence, USA). The cell concentration used for the proliferation assay [...]
Human non-small cell lung cancer (NSCLC) cell lines included the parental cell line A549 and the cell subline A549/DDP. Pt levels were expressed as μM/1 × 106 cells, with the cell number determined by counting (Nexcelom Cellometer Auto T4 cell counter, USA) in parallel cultures. Experiments were performed in duplicate and the values expressed were the means ± SD of the three independent experiments. […]
Gene expression actualizes the organismal phenotypes encoded within the genome in an environment-dependent manner. Among all encoded phenotypes, cell population growth rate (fitness) is perhaps the most important, since it determines how well-adapted a genotype is in various environments. Traditional biological measurement techniques have revealed the connection between the environment and fitness based on the gene expression mean. […]
Powell, E . et al. (2012) PLoS ONE. 7(2):e30993 doi:10.1371/journal.pone.0030993 -Trypan blue viability using Cellometer
Zuch, D., et al. (2012) J. Cell. Biochem. 113: 1282-1291. -Analysis of cell growth using Cellometer
Hong, C., et al. (2012). J. Clin Invest. 122(1): 337-347. -Trypan blue viability using Cellometer