Cellometer Vision Image Cytometer was used to capture and identify fluorescent positive GFP-LC3 cells. Autophagy is an important cellular catabolic process that plays a variety of important roles, including maintenance of the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearance of intracellular microbes. First, the damaged proteins, organelles or foreign microbes are isolated by double membrane vesicles called autophagosomes.  The vesicles then complete the enclosure of the damaged organelles and then fuses with lysosome. to form autolysosomes.  Finally, the materials are degraded within the autolysosomes and the nutrients are recycled back to the [...]

Ficoll separation is routinely used to isolate mononuclear cells from bone marrow, peripheral blood, and umbilical cord blood. Monocytes and lymphocytes form a buffy coat under a layer of plasma, where the cells are collected, then washed. Typically, some residual platelets and red blood cells are mixed in with the mononuclear cells. The degree of platelet and red blood cell contamination can vary significantly between donors and operators. The Cellometer Auto 2000 Cell Viability Counter utilizes dual fluorescence counting to generate accurate live cell and dead cell counts with no interference from red blood cells and platelets. Experimental Procedure Combine 20µl [...]

Isolation, Quantitation and Viability Analysis of Neonatal Cardiomyocytes using Cellometer The neonatal rat cardiomyocyte model has been used for many years by researchers studying the heart. In addition to increasing understanding of the morphological, biochemical, and electrophysiological characteristics of the normal heart, neonatal cardiomyocytes have been used to study contraction, ischemia, hypoxia, and the toxicity of different compounds. This model has been used to determine the optimal dosage for certain drugs and to develop and evaluate the efficacy of potential therapeutic agents. Apoptosis, or programmed cell death, has been observed in infarcted and re-perfused myocardium, end stages of heart failure, [...]

Apheresis is the process of removing a specific component of the blood, such as platelets, red blood cells, plasma (liquid part of the blood) or granulocytes (white blood cells) and returning the remaining components to the donor. Leukapheresis is a specific kind of apheresis in which white blood cells  are separated from a sample of blood. Cells contained in a leukapheresis product is mainly nucleated cells, some residual red blood cells and platelets. Application: measure live /dead nucleated cell concentration and viability Sample Type: human peripheral blood Leukapheresis Cell Type: total nucleated cells Staining Method: acridine orange and propidium iodide (Ao/PI) Instrument: [...]