What is adoptive cell transfer therapy? What cells are commonly used? What are the counting and viability methods for these cell types?
Webinar: The crucial need for accurate and consistent cell counting methods for development and manufacturing of CAR-T under GMP/GLP conditions.
Cellometer automated cell counters have been facilitating CAR T manufacturing with accurate cell counting and analysis since 2010.
Download our Poster Abstract: Cancer immunotherapy has been gaining momentum in the field of cancer research. Specifically, Chimeric Antigen Receptor (CAR) T cell technology have introduced new methods to combat cancer. Direct cell-mediated cytotoxicity assays are required to assess the killing capability of the engineered CAR T cells. Traditionally, these assays are conducted by measuring the amount of released Chromium, calcein AM, or LDH molecules after the target cancer cells are killed with CAR T cells. These methods require a large amount of target cells which may not be ideal when working with donor primary samples. Additionally, they cannot [...]
EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]
Emily Whitehead, cancer survivor, holding Nexcelom cell counting sheep. Thanks to the lifesaving T cell therapy clinical trial at Children’s Hospital of Philadelphia, Emily Whitehead is now two years cancer free. Diagnosed with acute lymphoblastic leukemia (ALL) right after her 5th birthday, doctors discovered that Emily’s leukemia was particularly resistant to chemotherapy, as are roughly 15% of the total number of ALL cases. After two recurrences of the disease, Emily’s parents enrolled her in a clinical trial for CTL019, an experimental therapeutic using a patient’s own reprogrammed T cells to eliminate the cancer cells. Now 9 years old, [...]
Patients with cancers such as multiple myeloma, leukemia, lymphoma, and other metastatic cancers have tumor cells with unique immunological targets that when exploited, can lead to the complete destruction of the mass and a lasting remission from disease. Clinicians attack those targets using cell therapy, also known as “targeted immunotherapy”. Cell therapy is the programming of a patient’s own immune cells to target that patient’s tumor cells for destruction. One application of cell therapy, Adoptive Cell Transfer (ACT), employs select methods of genetic manipulation and propagation of those immune cells ex vivo so that the newly programmed cells can be [...]
Leukocyte counts of BAL fluid BAL samples were mixed gently prior to diluting 50 ml BAL fluid with 50 ml of trypan blue (1:5 in 1xPBS) for viable cell counts. 20 ml of diluted cell sample were pipetted on a Cellometer counting chamber. Leukocytes were automatically counted using a Cellometer Auto T4 (Nexcelom Bioscience, Lawrence, MA, USA) […]
Viable T cells were enumerated every 7 days by trypan blue exclusion using the AutoT4 cell counter Cellometer (Nexcelom Bioscience, Lawrence, MA) […]
Canine adipose-derived mesenchymal stem cells (cASCs) When the monolayer of adherent cells reached approximately 70–80% confluence; trypsinization for cell splitting was performed using trypsin-EDTA solution (0.05%/0.02%, Biochrom AG, Berlin, Germany). After centrifugation, the cell pellets were subcultured at a concentration of 3 × 105cells/mL medium on 25cm2 tissue culture flasks containing DMEM/10% FCS. Cell counting was carried out by an automatic cell counter (Cellometer Nexcelom Bioscience, Lawrence, USA). The viable cell percentage from the harvested cells was calculated after trypan blue staining by an automatic cell counter (Cellometer Nexcelom Bioscience, Lawrence, USA). The cell concentration used for the proliferation assay [...]
