Do you have red-blood cells, cell debris, or tissue debris in your samples? When your samples contain unwanted particles use: Acridine Orange/ Propidium Iodide (AO/PI) viability assay to accurately measure the concentration and viability of your samples! AO/PI Protocol Obtain Nexcelom AO/PI solution: Cat # CS2-0106-5ML Stain cell sample at 1:1 with AO/PI solution Load counting chamber slide with 20 µl of cells and analyze Report Accurate and Consistent concentration and viability Results Do you have clean cell samples without RBCs, or other cell debris? When your samples are free from unwanted particles, Use Trypan Blue exclusion assay to accurately [...]

Perform image-based GFP analysis WITHOUT all of the set-up, maintenance, and data manipulation required for flow. Measure GFP Expression Efficiency Using Image Cytometry — In just a few clicks, Cellometer Vision CBA generates brightfield and fluorescent cell images, and detailed data reports. Detection of GFP expression to quantify the transfection/transduction efficiency. Single assay for measuring GFP expression and viability Measurement of other fluorescent proteins Detection of GFP Expression to Quantify the Transfection/Transduction Efficiency Quantitative measurement of GFP expression using Cellometer Load only 20 µl sample into counting chamber Capture and analyze brightfield and fluorescent cell images automatically Data [...]

Cellometer Vision Image Cytometer was used to capture and identify fluorescent positive GFP-LC3 cells. Autophagy is an important cellular catabolic process that plays a variety of important roles, including maintenance of the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearance of intracellular microbes. First, the damaged proteins, organelles or foreign microbes are isolated by double membrane vesicles called autophagosomes.  The vesicles then complete the enclosure of the damaged organelles and then fuses with lysosome. to form autolysosomes.  Finally, the materials are degraded within the autolysosomes and the nutrients are recycled back to the [...]