AO/PI Viability
Increase Accuracy, Remove Lysing Steps, and Optimize Productivity with Dual-Fluorescence AO/PI Staining and Multi-Parameter Viability Data From Bone Marrow, Cord Blood, Peripheral Blood, Tissue, and Other Sample Types
Jurkat Cells
Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available. Jurkat cells are widely used for IL-2 production and in the study of acute T cell leukemia, T cell signaling, and the effectiveness of potential anti-cancer compounds. Jurkat cells were used to establish the Nexcelom Apoptosis (Annexin V-FITC / PI) and PI Cell Cycle protocols for the Vision CBA [...]
Measure total nucleated cells collected by Apheresis
Apheresis is the process of removing a specific component of the blood, such as platelets, red blood cells, plasma (liquid part of the blood) or granulocytes (white blood cells) and returning the remaining components to the donor. Leukapheresis is a specific kind of apheresis in which white blood cells are separated from a sample of blood. Cells contained in a leukapheresis product is mainly nucleated cells, some residual red blood cells and platelets. Application: measure live /dead nucleated cell concentration and viability Sample Type: human peripheral blood Leukapheresis Cell Type: total nucleated cells Staining Method: acridine orange and propidium iodide (Ao/PI) Instrument: [...]
Trypan Blue Viability
Trypan Blue Viability: Viability is a measure of the metabolic state of a cell population which is indicative of the potential for growth. Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. Trypan blue dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging. Within 30 seconds, the Cellometer Auto T4 instrument counts both live cells and dead cells [...]
Hepatocytes
Hepatocytes are the predominant cell in the liver, comprising about 60% of liver cells and 80% of liver mass. One of the main hepatocyte functions is the production of bile. Hepatocytes are also responsible for the regulation of metabolism, detoxification, and the manufacture of important circulating proteins, making them a critical component of studies involving liver disease, drug metabolism, and toxicology. […]
ViaStain™ Viability Dyes
ViaStain™ Viability Dyes Nexcelom offers several dyes for fluorescence viability determination. AO (acridine orange) is a nuclear staining dye used to identify and count nucleated cells in a heterogeneous cell population. PI (propidium iodide) is a nuclear staining dye that only enters cells with compromised membranes. It is used to identify dead nucleated cells. AO/PI is a mixture of acridine orange and propidium iodide nuclear staining dyes. The dye mixture can be used with the Cellometer Auto 2000 and Cellometer Vision dual-fluorescence instruments to accurately count live and dead nucleated cells within heterogeneous samples containing non-nucleated cells, such as red [...]