About Leo Chan

Dr Leo Chan currently serves as the Technology R&D Manager at Nexcelom Bioscience LLC, Lawrence, MA. His research involves in the development of instrument and applications for the Celigo and Cellometer image cytometry system for detection and analysis of cells for oncology and immunology research. He is a member of the American Association of Cancer Research and the American Association of Immunologists. He received his B.S., M.S., and Ph.D. in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign (2000-2008).

Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells

It's White Paper Wednesday! This month's featured white paper: Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells Our understanding of autophagy has expanded tremendously in recent years, largely due to the identification of the many genes involved in the process, and the use of GFP-LC3 fusion proteins to visually monitor autophagosomes and autophagic activity both biochemically and microscopically [1, 2]. Recently, a novel fluorescent probe, Cyto-ID ® Green autophagy dye, has been developed to facilitate the investigation of the autophagic process [3-5]. In this study, a novel method was performed using the Cellometer image cytometry in combination with Cyto-ID Green autophagy dye for [...]

By |2021-06-15T20:40:28+00:00September 3rd, 2014|Categories: Cellometer, Cellometer Application News, Instrument|Tags: , , |0 Comments

Image cytometry method for autophagy activity detection in living cells

Cellometer Vision Image Cytometer was used to capture and identify fluorescent positive GFP-LC3 cells. Autophagy is an important cellular catabolic process that plays a variety of important roles, including maintenance of the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearance of intracellular microbes. First, the damaged proteins, organelles or foreign microbes are isolated by double membrane vesicles called autophagosomes.  The vesicles then complete the enclosure of the damaged organelles and then fuses with lysosome. to form autolysosomes.  Finally, the materials are degraded within the autolysosomes and the nutrients are recycled back to the [...]

By |2021-06-15T20:44:50+00:00June 10th, 2013|Categories: Cell Counting Leadership, Cellometer Application News|0 Comments
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