Leo Chan, Author at Nexcelom Bioscience

The Historical Development of the Hemacytometer

It's White Paper Wednesday! Read our featured white paper:The Historical Development of the Hemacytometer The hemacytometer has been an essential tool for hematologists, medical practitioners, and biologists for over a century. Depending on where it is being used, the word has multiple spellings such as hemacytometer, hemocytometer, haemacytometer, or haemocytometer, but for consistency purposes the word “hemacytometer” will be used in this review. The prefix “hema”, “hemo”, “haema”, or “haemo” means blood, while “cytometer” meant a device to measure cells. The device was initially used by medical practitioners to analyze patient blood samples, which was the initial spark that created [...]

By Leo Chan|2021-06-15T20:34:42+00:00February 11th, 2015|Categories: Cell Counting Leadership, Cellometer Application News|Tags: hemacytometer, Hemocytometer, History, White Paper|0 Comments

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By Leo Chan|2021-06-15T20:35:02+00:00January 29th, 2015|Categories: Cellometer User Publications, Immuno-oncology|Tags: Apoptosis, Cell Cycle, Cellometer Vision, Ovarian Cancer|0 Comments

Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

By Leo Chan|2021-06-15T20:35:18+00:00January 22nd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autophagosomes, Autophagy, Cell Cycle, Cellometer Vision, Cyto-ID, Proliferation|0 Comments

Cellometer Vision CBA image cytometry reveals the utility of the immunocompetent mouse model “Glowing Head” in assessing preclinical anti-tumor therapies

Introduction Preclinical models to evaluate therapeutic efficacy in the area of oncology have proven to be a challenge. The standard of preclinical assessment as always been immunosuppressed mice, but that model has not reliably predicted clinical efficacy [1]. Immunocompetent genetically engineered mice with tumor allograft models seem more promising, and yet reporter tags such as GFP and luciferase are targeted by competent immune systems, which may interfere with tumor growth and the response to therapeutics. Here, a collaborative group of researchers has created a reporter-tolerized genetically engineered mouse model, dubbed “Glowing Head” (GH), by targeting a luciferase-GFP reporter into the [...]

By Leo Chan|2021-06-15T20:35:29+00:00January 15th, 2015|Categories: Animal Models, Cellometer, Cellometer User Publications, Instrument|Tags: anti-tumor therapies, Cellometer Vision CBA, Glowing Head, Luciferase-GFP reporter|0 Comments

Iron oxide-based nanoparticles conjugated NuBCP-9 increase MCF-7 apoptosis as assessed by Cellometer Vision CBA Image Cytometer

Introduction The B-cell lymphoma-2 (BCL-2) family of proteins includes many key regulators of cell survival, cell death, and apoptosis. The deregulation of certain BCL-2 proteins is one of the first steps towards tumorigenesis and subsequent therapeutic resistance [1-4]. Targeting these BCL-2 family members is therefore an important area of oncology research [2, 4, 5]. Here, a collaboration of researchers has explored the targeting of these BCL-2 proteins with a BCL-2 binding peptide known as NuBCP-9 (FSRSLHSLL), which induces a conformational change in BCL-2 that alters the protein’s function from cell protection to cell killing. Researchers paired NuBCP-9 with iron oxide-based [...]

By Leo Chan|2021-06-15T20:35:46+00:00January 8th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Apoptosis, Cellometer Vision CBA|0 Comments

Investigation of IAPP Role in Increasing ROS Production and Apoptosis in p53-deficient Tumor Cells using Celigo Imaging Cytometer

The entire family of tumor protein p53 (TP53) enhances functions such as apoptosis and autophagy in normal cellular functioning. TP53 is a tumor repressor gene that is often inactivated in human cancers. Reactivating p53 has proven difficult to achieve therapeutically, however. Researchers at MD Anderson Cancer Center are investigating other members of the p53 pathway in order to elucidate new therapeutic options to suppress p53-deficient tumor growth. ΔN isoforms of two members of the p53 family, p63 and p73, are usually overexpressed in cancers and these isoforms (which lack the acidic transactivation domain) act on p53 in a dominant-negative fashion, [...]

By Leo Chan|2021-06-15T20:36:01+00:00December 23rd, 2014|Categories: Celigo, Celigo User Publications, Instrument|Tags: Imaging Cytometry, tumor protein p53, tumor protein p63, tumor protein p73, Tumor Survival|0 Comments

Evaluation of Nanoparticle-Based Therapy for Triple-Negative Breast Cancer using Cellometer Vision CBA

Introduction to Nanoparticle-Based Therapy for Triple-Negative Breast Cancer Tia Harmon and Dr. Ruben Gonzalez-Perez from the Morehouse School of Medicine and Emory University utilized the imaging and analysis capabilities of the Cellometer Vision to investigate leptin peptide receptor antagonist-conjugated nanoparticles in order to inhibit leptin signaling, a key pathway that promotes growth and survival of triple-negative breast cancer (TNBC) cells. It was hypothesized that these nanoparticles would first stop the expression of leptin’s downstream target Notch, and would thereby increase the effectiveness of standard chemotherapeutic compounds in limiting tumor cell survival [1-4]. Materials and Methods A human ER+ cell line (MCF-7) [...]

By Leo Chan|2021-06-15T20:36:25+00:00December 8th, 2014|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|0 Comments

Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation

It’s White Paper Wednesday! This month’s featured white paper: Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation The hemacytometer persists as the gold standard for laboratory cell counting. First utilized in 18th century France as a means to analyze patient blood samples, the hemacytometer has gone through a series of major developments over the past hundreds of years, creating a modern instrument that is more accurate and easier to use than its predecessors. The hemacytometer remains an integral part of all cell-based research, and yet sources of error inherent in its design and utilization persist. Those sources [...]

By Leo Chan|2021-06-15T20:36:59+00:00November 12th, 2014|Categories: Cell Counting Leadership, Cellometer Application News|Tags: Cell Counting, hemacytometer, Hemocytometer|0 Comments

Rapid Image Cytometry Method for Cell Counting and Viability Measurement for Cellular Therapy

Patients with cancers such as multiple myeloma, leukemia, lymphoma, and other metastatic cancers have tumor cells with unique immunological targets that when exploited, can lead to the complete destruction of the mass and a lasting remission from disease. Clinicians attack those targets using cell therapy, also known as “targeted immunotherapy”. Cell therapy is the programming of a patient’s own immune cells to target that patient’s tumor cells for destruction. One application of cell therapy, Adoptive Cell Transfer (ACT), employs select methods of genetic manipulation and propagation of those immune cells ex vivo so that the newly programmed cells can be [...]

By Leo Chan|2021-06-15T20:37:41+00:00October 13th, 2014|Categories: CAR T / Cell Therapy, Cellometer Application News, Instrument|Tags: Cell Counting, Cellular Therapy, image cytometry|0 Comments

Accurately Count PBMC and Measure Viability in Presence of Residual RBC

It’s White Paper Wednesday! This month’s featured white paper: Accurately Count PBMC and Measure Viability in Presence of Residual RBC In this work, we have developed an image cytometry method for detecting and monitoring the cell expansion and differentiation of articular chondrocytes in primary culture. First, the feasibility of utilizing image cytometry for detection of fluorescent is shown by comparing measured fluorescent positive cell populations to flow cytometry. Next, articular chondrocyte cultures were established in multi-well plates from either single or Cyan/eGFP double reporter mouse lines and grown for 20 days to test the utility of the fluorescence-based image cytometry system. [...]

By Leo Chan|2021-06-15T20:39:15+00:00October 1st, 2014|Categories: Cell Counting Leadership, Cellometer Application News|Tags: PBMCs, RBC Contamination, White Paper|0 Comments

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