Channou Aing, Author at Nexcelom Bioscience

Analytical Biochemistry 2016

Read more Cellometer image cytometry as a complementary tool to flow cytometry for verifying gated cell populations Analytical Biochemistry Dmitry Kuksin, Christina Arieta Kuksin, Jean Qiu, Leo Li-Ying Chan Traditionally, many cell-based assays that analyze cell populations and functionalities have been performed using flow cytometry. However, flow cytometers remain relatively expensive and require highly trained operators for routine maintenance and data analysis. Recently, an image cytometry system has been developed by Nexcelom Bioscience (Lawrence, MA, USA) for automated cell concentration and viability measurement using bright-field and fluorescent imaging methods. Image cytometry is analogous to flow cytometry in that gating operations can [...]

By Channou Aing|2021-03-25T18:33:33+00:00March 25th, 2021|0 Comments

Cytotechnology 2016

Read more A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry Cytotechnology Leo Li-Ying Chan,Tim Smith, Kendra A. Kumph, Dmitry Kuksin, Sarah Kessel, Olivier Déry, Scott Cribbes, Ning Lai, Jean Qiu To ensure cell-based assays are performed properly, both cell concentration and viability have to be determined so that the data can be normalized to generate meaningful and comparable results. Cell-based assays performed in immuno-oncology, toxicology, or bioprocessing research often require measuring of multiple samples and conditions, thus the current automated cell counter that uses single disposable counting slides is not practical for high-throughput screening assays. [...]

By Channou Aing|2021-03-25T18:33:11+00:00March 25th, 2021|0 Comments

SAGE Journals 2017

Read more A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer SAGE Journals Scott Cribbes, Sarah Kessel, Scott McMenemy, Jean Qiu, Leo Li-Ying Chan Three-dimensional (3D) tumor models have been increasingly used to investigate and characterize cancer drug compounds. The ability to perform high-throughput screening of 3D multicellular tumor spheroids (MCTS) can highly improve the efficiency and cost-effectiveness of discovering potential cancer drug candidates. Previously, the Celigo Image Cytometer has demonstrated a novel method for high-throughput screening of 3D multicellular tumor spheroids. In this work, we employed the Celigo Image Cytometer to [...]

By Channou Aing|2021-03-25T18:32:57+00:00March 25th, 2021|0 Comments

Journal of Industrial Microbiology and Biotechnology 2017

Read more A novel concentration and viability detection method for Brettanomyces using the Cellometer image cytometry Journal of Industrial Microbiology and Biotechnology. Martyniak B, Bolton J, Kuksin D, Shahin SM, Chan LL. Brettanomyces spp. can present unique cell morphologies comprised of excessive pseudohyphae and budding, leading to difficulties in enumerating cells. The current cell counting methods include manual counting of methylene blue-stained yeasts or measuring optical densities using a spectrophotometer. However, manual counting can be time-consuming and has high operator-dependent variations due to subjectivity. Optical density measurement can also introduce uncertainties where instead of individual cells counted, an average of a [...]

By Channou Aing|2021-03-25T18:32:43+00:00March 25th, 2021|0 Comments

Methods in Molecular Biology 2017

Read more Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry Methods in Molecular Biology. Chan LL, McCulley KJ, Kessel SL The ability to accurately measure cell viability is important for any cell-based assay. Traditionally, viability measurements have been performed using the trypan blue exclusion method on a hemacytometer, which allows researchers to visually distinguish viable from nonviable cells. While the trypan blue method can work for cell lines or primary cells that have been rigorously purified, in more complex samples such as PBMCs, bone marrow, whole blood, or any sample with low viability, this method can lead to [...]

By Channou Aing|2021-03-25T18:32:19+00:00March 25th, 2021|0 Comments

Journal of Immunological Methods 2017

Read more Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer Journal of Immunological Methods.Zhang H, Chan LL, Rice W, Kassam N, Longhi MS, Zhao H, Robson SC, Gao W, Wu Y Hybridoma screening is a critical step for antibody discovery, which necessitates prompt identification of potential clones from hundreds to thousands of hybridoma cultures against the desired immunogen. Technical issues associated with ELISA- and flow cytometry-based screening limit accuracy and diminish high-throughput capability, increasing time and cost. Conventional ELISA screening with coated antigen is also impractical for difficult-to-express hydrophobic membrane antigens or multi-chain protein complexes. Here, we demonstrate novel high-throughput screening methodology employing the Celigo Image [...]

By Channou Aing|2021-03-25T18:32:04+00:00March 25th, 2021|0 Comments

Cytometry Part A 2017

Read more Real-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image Cytometer Cytometry Part A. Kessel S, Cribbes S, Bonasu S, Rice W, Qiu J, Chan LL. The development of three-dimensional (3D) multicellular tumor spheroid models for cancer drug discovery research has increased in the recent years. The use of 3D tumor spheroid models may be more representative of the complex in vivo tumor microenvironments in comparison to two-dimensional (2D) assays. Currently, viability of 3D multicellular tumor spheroids has been commonly measured on standard plate-readers using metabolic reagents such as CellTiter-Glo® for end point analysis. Alternatively, high content image [...]

By Channou Aing|2021-03-25T18:31:48+00:00March 25th, 2021|0 Comments

Cancer Research 2017

Read more Optical Coherence Tomography Detects Necrotic Regions and Volumetrically Quantifies Multicellular Tumor Spheroids Cancer Research. Huang Y, Wang S, Guo Q, Kessel S, Rubinoff I, Chan LL, Li P, Liu Y, Qiu J, Zhou C Three-dimensional (3D) tumor spheroid models have gained increased recognition as important tools in cancer research and anticancer drug development. However, currently available imaging approaches used in high-throughput screening drug discovery platforms, for example, bright-field, phase contrast, and fluorescence microscopies, are unable to resolve 3D structures deep inside (>50 μm) tumor spheroids. In this study, we established a label-free, noninvasive optical coherence tomography (OCT) imaging platform to characterize 3D morphologic and physiologic information of multicellular [...]

By Channou Aing|2021-03-25T18:31:28+00:00March 25th, 2021|0 Comments

SLAS Discovery 2018

Read more Real-Time Apoptosis and Viability High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer SLAS Discovery. Kessel S, Cribbes S, Bonasu S, Qiu J, Chan LL. Three-dimensional tumor spheroid models have been increasingly used to investigate and characterize cancer drug compounds. Previously, the Celigo image cytometer has demonstrated its utility in a high-throughput screening manner for evaluating potential drug candidates in a 3D multicellular tumor spheroid (MCTS) primary screen. In addition, we have developed real-time kinetic caspase 3/7 apoptosis and propidium iodide viability 3D MCTS assays, both of which can be used in a secondary screen to better characterize the hit [...]

By Channou Aing|2021-03-25T18:30:56+00:00March 25th, 2021|0 Comments

Trypan Blue vs. Fluorescent Cell Counting and Viability Webinar

https://youtu.be/ECngzJ9FQvg Video: Trypan Blue vs. Fluorescent Cell Counting and Viability Webinar Are you using the right method? A few takeaways from this webinar is: FDA requires more confidence in cell counting measurements, ISO has published two cell counting standards to guide the selection and evaluation of cell counting measurement, RBC contamination can affect live cell counts with bright field + TB, Trypan blue can damage cells beyond quantification, which contributes to higher viability results, Replace trypan blue with fluorescent dyes for better viability measure, and Image Cytometers are designed to enable more cell characterization assays.

By Channou Aing|2021-04-14T19:06:14+00:00March 17th, 2021|0 Comments

About Channou Aing