Channou Aing, Author at Nexcelom Bioscience

J Transl Med. 2014 Aug 21;12(1):226

Read more Evaluation of the antitumor effects of c-Myc-Max heterodimerization inhibitor 100258-F4 in ovarian cancer cells J Transl Med. 2014 Aug 21;12(1):226. Wang J, Ma X, Jones HM, Chan L, Song F, Zhang W, Bae-Jump VL, Zhou C. Epithelial ovarian carcinoma is the most lethal gynecological cancer due to its silent onset and recurrence with resistance to chemotherapy. Overexpression of oncogene c-Myc is one of the most frequently encountered events present in ovarian carcinoma. Disrupting the function of c-Myc and its downstream target genes is a promising strategy for cancer therapy. Our objective was to evaluate the potential effects of small-molecule [...]

By Channou Aing|2021-03-25T18:53:35+00:00March 25th, 2021|0 Comments

J. Am. Soc. Brew. Chem. 72(4):253-260, 2014

Read more Image-Based Cytometric Analysis of Fluorescent Viability and Vitality Staining Methods for Ale and Lager Fermentation Yeast J. Am. Soc. Brew. Chem. 72(4):253-260, 2014. Stephanie Saldi, Department of Technology R&D, Nexcelom Bioscience LLC., Lawrence, MA 01843; Dan Driscoll, Avery Brewing Company, 5763 Arapahoe Ave. Ste. E, Boulder, CO 80303; and Dmitry Kuksin and Leo Li-Ying Chan, Department of Technology R&D, Nexcelom Bioscience LLC., Lawrence, MA 01843. Saccharomyces cerevisiae have been an essential component of beer production for centuries. The viability and vitality of yeast during a fermentation brewing process is an especially important consideration for proper cell growth, consistent flavor, and [...]

By Channou Aing|2021-03-25T18:51:31+00:00March 25th, 2021|0 Comments

Oncotarget 2015

Read more JQ1 suppresses tumor growth through downregulating LDHA in ovarian cancer Oncotarget Qiu H, Jackson AL, Kilgore JE, Zhong Y, Chan LL, Gehrig PA, Zhou C2, Bae-Jump VL. Amplification and overexpression of c-Myc is commonly seen in human ovarian cancers, and this could be a potentially novel therapeutic target for this disease. JQ1, a selective small-molecule BET bromodomain (BRDs) inhibitor, has been found to suppress tumor progression in several cancer cell types. Using ovarian cancer cell lines, a transgenic mouse model, and primary cell cultures from human ovarian cancer tissues, we demonstrated that JQ1 significantly suppressed cellular proliferation and induced [...]

By Channou Aing|2021-03-25T18:49:13+00:00March 25th, 2021|0 Comments

PLoS One Somanchi SS 2015

Read more A Novel Method for Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry PLoS One Somanchi SS, McCulley KJ, Somanchi A, Chan LL, Lee DA. Natural killer (NK) cells belong to the innate arm of the immune system and though activated NK cells can modulate immune responses through the secretion of cytokines, their primary effector function is through target cell lysis. Accordingly, cytotoxicity assays are central to studying NK cell function. The 51Chromium release assay, is the “gold standard” for cytotoxicity assay, however, due to concerns over toxicity associated with the use and disposal of radioactive compounds there is [...]

By Channou Aing|2021-03-25T18:47:12+00:00March 25th, 2021|0 Comments

Journal of College Biology Teaching 2015

Read more Investigation of Macrophage Differentiation and Cytokine Production in an Undergraduate Immunology Laboratory Journal of College Biology Teaching Charlotte Berkes and Leo Li-Ying Chan We have developed a semester-long laboratory project for an undergraduate immunology course in which students study multiple aspects of macrophage biology including differentiation from progenitors in the bone marrow, activation upon stimulation with microbial ligands, expression of cell surface markers, and modulation of cytokine production. In the first part of the semester, students differentiate macrophages from mouse bone marrow stem cells and perform immunophenotyping on their macrophages using myeloid markers that are either constitutively expressed or [...]

By Channou Aing|2021-03-25T18:44:57+00:00March 25th, 2021|0 Comments

MBAA TQ 2016

Read more Measuring Lager and Ale Yeast Viability and Vitality Using Fluorescence-Based Image Cytometry MBAA TQ Leo Li-Ying Chan, Dan Driscoll, Dmitry Kuksin, and Stephanie Saldi Saccharomyces cerevisiae has been indispensable to the production of beer for generations. Throughout the brewing process, establishing proper yeast viability and vitality is a crucial concern for proper cell growth, optimal yield, and flavor consistency. Viable yeast are those with intact membranes, whereas vital yeast are those cells with quantifiable metabolic activity and the capacity to proliferate. It is possible to have yeast that are viable but not actively propagating, and this adversely affects the fermentation [...]

By Channou Aing|2021-03-25T18:43:22+00:00March 25th, 2021|0 Comments

Natural Killer Cells 2016

Read more Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry Method Natural Killer Cells Kelsey J. McCulley and Srinivas S. Somanchi Although natural killer (NK) cells produce various cytokines that regulate other lymphocytes of the immune system, the primary effector function of NK cells is the direct cytolysis of their targets. Hence analyzing the cytotoxic potential of these lymphocytes is fundamental to understanding their biology and their clinical impact. We have previously shown that release-based cytotoxicity assays, such as calcein release assay, could potentially underestimate percent specific lysis if the entrapped reporter is not completely released and demonstrated that an [...]

By Channou Aing|2021-03-25T18:41:14+00:00March 25th, 2021|0 Comments

Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging 2016

Read more A Rapid Method for Detecting Autophagy Activity in Live Cells Using Cellometer Image Cytometry Autophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging Leo Li-Ying Chan, Ning Lai, Dee Shen, Alisha R. Wilkinson, Wayne Patton, Eric Chan, Dmitry Kuksin, Bo Lin and Jean Qiu Autophagy is a highly regulated and complex process that plays an important role in cellular functions such as maintaining the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearing intracellular microbes. The current autophagy detection methods include fluorescence microscopy, biochemical measurement, SDS-PAGE, and Western blotting, but they are time-consuming, labor-intensive, and [...]

By Channou Aing|2021-03-25T18:39:04+00:00March 25th, 2021|0 Comments

MBAA TQ 2016

Read more Measuring Glycogen, Neutral Lipid, and Trehalose Contents in Yeast Using Fluorescence- Based Image Cytometry MBAA TQ Leo Li-Ying Chan, Alexandria Kury, Alisha Wilkinson, Charlotte Berkes, and Alnoor Pirani The brewing, baking, and biofuel industries have long been involved with monitoring the physiological and metabolic changes of Saccharomyces cerevisiae. Breweries in particular need to observe cell health parameters such as viability, vitality, and contents of glycogen, neutral lipid, and trehalose to assess yeast cells over the course of fermentation. These physiological and metabolic variations can be qualitatively maintained via fluorescence microscopy or quantitatively evaluated via flow cytometry, using fluorescently labeled [...]

By Channou Aing|2021-03-25T18:36:45+00:00March 25th, 2021|0 Comments

Journal of Laboratory Automation 2016

Read more High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry Journal of Laboratory Automation Sarah Kessel, Scott Cribbes, Olivier Déry, Dmitry Kuksin, Oncologists have investigated the effect of protein or chemical-based compounds on cancer cells to identify potential drug candidates. Traditionally, the growth inhibitory and cytotoxic effects of the drugs are first measured in 2D in vitro models, and then further tested in 3D xenograft in vivo models. Although the drug candidates can demonstrate promising inhibitory or cytotoxicity results in a 2D environment, similar effects may not be observed under a 3D environment. In this [...]

By Channou Aing|2021-03-25T18:33:48+00:00March 25th, 2021|0 Comments

About Channou Aing