Channou Aing, Author at Nexcelom Bioscience

J Immunol Methods. 2013 Feb 28

Read more Accurate Measurement of Peripheral Blood Mononuclear Cell Concentration using Image Cytometry to Eliminate RBC-induced Counting Error J Immunol Methods. 2013 Feb 28, Chan LL, Laverty DJ, Smith T, Nejad P, Hei H, Gandhi R, Kuksin D, Qiu J. Peripheral blood mononuclear cells (PBMCs) have been widely researched in the fields of immunology, infectious disease, oncology, transplantation, hematological malignancy, and vaccine development. Specifically, in immunology research, PBMCs have been utilized to monitor concentration, viability, proliferation, and cytokine production from immune cells, which are critical for both clinical trials and biomedical research. The viability and concentration of isolated PBMCs are traditionally [...]

By Channou Aing|2021-03-25T19:16:26+00:00March 25th, 2021|0 Comments

J Ind Microbiol Biotechnol. 2013 Apr 5

Read more Automated Quantification of Budding Saccharomyces Cerevisiae using a Novel Image Cytometry Method J Ind Microbiol Biotechnol. 2013 Apr 5, Laverty DJ, Kury AL, Kuksin D, Pirani A, Flanagan K, Chan LL. The measurements of concentration, viability, and budding percentages of Saccharomyces cerevisiae are performed on a routine basis in the brewing and biofuel industries. Generation of these parameters is of great importance in a manufacturing setting, where they can aid in the estimation of product quality, quantity, and fermentation time of the manufacturing process. Specifically, budding percentages can be used to estimate the reproduction rate of yeast populations, which [...]

By Channou Aing|2021-03-25T19:14:16+00:00March 25th, 2021|0 Comments

Autophagy. 2013 May;9(5):683-96

Read more Acknowledgement: Type I interferons induce autophagy in certain human cancer cell lines Autophagy. 2013 May;9(5):683-96. Schmeisser H, Fey SB, Horowitz J, Fischer ER, Balinsky CA, Miyake K, Bekisz J, Snow AL, Zoon KC. Autophagy is an evolutionarily conserved cellular recycling mechanism that occurs at a basal level in all cells. It can be further induced by various stimuli including starvation, hypoxia, and treatment with cytokines such as IFNG/IFNγ and TGFB/TGFβ. Type I IFNs are proteins that induce an antiviral state in cells. They also have antiproliferative, proapoptotic and immunomodulatory activities. We investigated whether type I IFN can also induce [...]

By Channou Aing|2021-03-25T19:12:04+00:00March 25th, 2021|0 Comments

JOVE 2013

Read more Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells JOVE. Charlotte Berkes, Leo Li-Ying Chan, Alisha Wilkinson, Benjamin Paradis Studies of the cellular pathogenesis mechanisms of pathogenic yeasts such as Candida albicans, Histoplasma capsulatum, and Cryptococcus neoformans commonly employ infection of mammalian hosts or host cells (i.e. macrophages) followed by yeast quantification using colony forming unit analysis or flow cytometry. While colony forming unit enumeration has been the most commonly used method in the field, this technique has disadvantages and limitations, including slow growth of some fungal species on solid media and low and/or [...]

By Channou Aing|2021-03-25T19:09:51+00:00March 25th, 2021|0 Comments

Cancer and Metabolism 2013

Read more The Metabolic Demands of Cancer Cells are Coupled to their Size and Protein Synthesis Rates Cancer & Metabolism. Sonia C Dolfi, Leo Li-Ying Chan, Jean Qiu, Philip M Tedeschi, Joseph R Bertino, Kim M Hirshfield, Zoltán N Oltvai and Alexei Vazquez Although cells require nutrients to proliferate, most nutrient exchange rates of the NCI60 panel of cancer cell lines correlate poorly with their proliferation rate. Here, we provide evidence indicating that this inconsistency is rooted in the variability of cell size.

By Channou Aing|2021-03-25T19:08:05+00:00March 25th, 2021|0 Comments

Cell Death Dis. 2013 Oct 24

Read more Contribution of Serine, Folate and Glycine Metabolism to the ATP, NADPH and Purine Requirements of Cancer Cells Cell Death Dis. 2013 Oct 24, Tedeschi PM, Markert EK, Gounder M, Lin H, Dvorzhinski D, Dolfi SC, Chan LL, Qiu J, Dipaola RS, Hirshfield KM, Boros LG, Bertino JR, Oltvai ZN, Vazquez A. Recent observations on cancer cell metabolism indicate increased serine synthesis from glucose as a marker of poor prognosis. We have predicted that a fraction of the synthesized serine is routed to a pathway for ATP production. The pathway is composed by reactions from serine synthesis, one-carbon (folate) [...]

By Channou Aing|2021-03-25T19:05:44+00:00March 25th, 2021|0 Comments

Cytotechnology. 2014 Mar 19

Read more Morphological Observation and Analysis using Automated Image Cytometry for the Comparison of Trypan Blue and Fluorescence-based Viability Detection Method Cytotechnology. 2014 Mar 19. Chan LL1, Kuksin D, Laverty DJ, Saldi S, Qiu J. The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement method has been the trypan blue (TB) exclusion assay. However, since the introduction of fluorescence-based dyes for cell viability measurement using flow or image-based cytometry systems, [...]

By Channou Aing|2021-03-25T19:03:39+00:00March 25th, 2021|0 Comments

J Fluoresc. 2014 Apr 13

Read more Discriminating Multiplexed GFP Reporters in Primary Articular Chondrocyte Cultures Using Image Cytometry J Fluoresc. 2014 Apr 13. Chan LL, Huang J, Hagiwara Y, Aguila L, Rowe D. Flow cytometry has become a standard tool for defining a heterogeneous cell population based on surface expressed epitopes or GFP reporters that reflect cell types or cellular differentiation. The introduction of image cytometry raised the possibility of adaptation to discriminate GFP reporters used to appreciate cell heterogeneity within the skeletal lineages. The optical filters and LEDs were optimized for the reporters used in transgenic mice expressing various fluorescent proteins. In addition, the [...]

By Channou Aing|2021-03-25T19:01:40+00:00March 25th, 2021|0 Comments

J Fluoresc. 2014 Apr 17

Read more Automated Enumeration and Viability Measurement of Canine Stromal Vascular Fraction Cells Using Fluorescence-Based Image Cytometry Method J Fluoresc. 2014 Apr 17. Chan LL, Cohen DA, Kuksin D, Paradis BD, Qiu J. In recent years, the lipoaspirate collected from adipose tissue has been seen as a valuable source of adipose-derived mesenchymal stem cells for autologous cellular therapy [1-3]. For multiple applications, adipose-derived mesenchymal stem cells are isolated from the stromal vascular fraction (SVF) of adipose tissue. Because the fresh stromal vascular fraction typically contains a heterogeneous mixture of cells [4, 5], determining cell concentration and viability is a crucial step [...]

By Channou Aing|2021-03-25T18:59:33+00:00March 25th, 2021|0 Comments

Journal of the ASBC 72(2):102-109, 2014

Read more Acknowledgement: A Novel Method of Inducing and Retaining Cell Cycle Synchronization in Cultures of Saccharomyces cerevisiae Journal of the ASBC 72(2):102-109, 2014. J. Blake Layfield, Lucas R. Vann, and John D. Sheppard In conventional fermentation, at any one time, individual yeast cells are randomly distributed with respect to the stage of their growth and division cycle. The observed metabolic performance is, therefore, the result of an average of the entire population. In contrast, a synchronous population is characterized by cells that are aligned with respect to their metabolic processes, traversing the cell cycle and dividing mostly in unison. In this [...]

By Channou Aing|2021-03-25T18:57:02+00:00March 25th, 2021|0 Comments

About Channou Aing