Channou Aing, Author at Nexcelom Bioscience

Poster: Celigo using AOPI Fluorescent Stains

Download the Poster Image Cytometry-Based High-Throughput Cell Concentration and Viability Detection Method using AOPI Fluorescent Stains Leo L. Chan, Tim Smith, Kendra A. Kumph, Dmitry Kuksin, Sarah Kessel, Olivier Déry, Scott Cribbes, Ning Lai and Jean Qiu Cell concentration and viability measurement are two although simple but highly important parameters to ensure cell-based assays are performed properly. These two parameters have to be determined so that the final data can be normalized to generate meaningful and comparable results for downstream experiments. Cell-based assays performed in immuno-oncology, toxicology, or bioprocessing research fields often require measuring of multiple samples and conditions, thus [...]

By Channou Aing|2021-04-23T18:28:51+00:00March 30th, 2021|0 Comments

Journal of Industrial Microbiology Biotechnology 2010

Read more Direct Concentration and Viability Measurement of Yeast in Corn Mash using a Novel Imaging Cytometry Method Journal of Industrial Microbiology Biotechnology, October 2010. Chan LL, Lyettefi EJ, Pirani A, Smith T, Qiu J, Lin B. Worldwide awareness of fossil-fuel depletion and global warming has been increasing over the last 30 years. Numerous countries, including the USA and Brazil, have introduced large-scale industrial fermentation facilities for bioethanol, biobutanol, or biodiesel production. Most of these biofuel facilities perform fermentation using standard baker’s yeasts that ferment sugar present in corn mash, sugar cane, or other glucose media. In research and development in [...]

By Channou Aing|2021-03-25T19:35:47+00:00March 25th, 2021|0 Comments

Journal of Pharmacological and Toxicological Methods 2010

Read more Rapid Detection of ABC Transporter Interaction: Potential Utility in Pharmacology Journal of Pharmacological and Toxicological Methods, November 2010. Robey RW, Lin B, Qiu J, Chan LL, Bates SE We have developed a method to characterize the function and inhibition of ABC transporters using an automated cell counter with fluorescence detection capability. The assay was performed using stably-transfected HEK293 cells expressing P-gp, MRP1, or ABCG2 and examining transport of fluorescent substrates in the presence or absence of known inhibitors and compared to results obtained with a flow cytometer.

By Channou Aing|2021-03-25T19:33:33+00:00March 25th, 2021|0 Comments

Cytometry Part A 2011

Read more Cellometer Vision as an Alternative to Flow Cytometry for Cell Cycle Analysis, Mitochondrial Potential, and Immunophenotyping Cytometry Part A, April 2011. Leo L. Chan, Xuemei Zhong, Jean Qiu, Peter Y. Li, Bo Lin Cell phenotyping and cell cycle analysis are two commonly used assays in both clinical diagnosis and biomedical research. Cell phenotyping by identifying different biomarkers is essential for the diagnosis of hematologic malignancy, sub-classifying diseases, monitoring response to treatment, predicting prognosis, detecting rare cell populations and residual malignant cells.

By Channou Aing|2021-03-25T19:31:59+00:00March 25th, 2021|0 Comments

Apoptosis 2012

Read more A Rapid Detection Method for Apoptosis and Necrosis Measurement using the Cellometer Imaging Cytometry Apoptosis, December 2011. Chan LL, Lai N, Wang E, Smith T, Yang X, Lin B. Apoptosis and necrosis play an important role in various aspects of preclinical pharmaceutical drug discovery and validation. The ability to quickly determine the cytotoxic effect of chemical compounds on cancer cells allows researchers to efficiently identify potential drug candidates for further development in the pharmaceutical discovery pipeline. Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis.

By Channou Aing|2021-03-25T19:29:58+00:00March 25th, 2021|0 Comments

J Immunol Methods 2012

Read more A Novel Method for Kinetic Measurements of Rare Cell Proliferation using Cellometer Image-Based Cytometry J Immunol Methods, March 2012. Chan LL, Zhong X, Pirani A, Lin B. Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines. For immunological studies, the ability to perform rapid cell proliferation analysis allows the identification of potential biological reagents for inducing or inhibiting immune cell proliferation. Current cell proliferation analysis methods employ flow cytometry for fluorescence detection of CFSE-labeled cells. However, conventional flow cytometers require a [...]

By Channou Aing|2021-03-25T19:28:15+00:00March 25th, 2021|0 Comments

Society for Industrial Microbiology and Biotechnology 2012

Read more Novel Image Cytometric Method for Detection of Physiological and Metabolic Changes in Saccharomyces cerevisiae Society for Industrial Microbiology and Biotechnology, August 2012. Chan LL, Kury A, Wilkinson A, Berkes C, Pirani A. The studying and monitoring of physiological and metabolic changes in Saccharomyces cerevisiae (S. cerevisiae) has been a key research area for the brewing, baking, and biofuels industries, which rely on these economically important yeasts to produce their products. Specifically for breweries, physiological and metabolic parameters such as viability, vitality, glycogen, neutral lipid, and trehalose content can be measured to better understand the status of S. cerevisiae during [...]

By Channou Aing|2021-03-25T19:26:20+00:00March 25th, 2021|0 Comments

Autophagy, September 2012

Read more A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells Autophagy, September 2012. Leo Li-Ying Chan, Dee Shen, Alisha R. Wilkinson, Wayne Patton, Ning Lai, Eric Chan, Dmitry Kuksin, Bo Lin and Jean Qiu Autophagy is an important cellular catabolic process that plays a variety of important roles, including maintenance of the amino acid pool during starvation, recycling of damaged proteins and organelles, and clearance of intracellular microbes. Currently employed autophagy detection methods include fluorescence microscopy, biochemical measurement, SDS-PAGE and western blotting, but they are time consuming, labor intensive, and require much experience for accurate interpretation. More recently, [...]

By Channou Aing|2021-03-25T19:24:31+00:00March 25th, 2021|0 Comments

Journal of fluorescence, September 2012

Read more Rapid Image-based Cytometry for Comparison of Fluorescent Viability Staining Methods Journal of fluorescence, September 2012. Chan LL, Wilkinson AR, Paradis BD, Lai N. The ability to accurately measure cell viability is important for any cell-based research. Traditionally, viability measurements have been performed using trypan blue exclusion method on hemacytometer, which allowed researchers to visually distinguish viable from nonviable cells. However, the trypan blue method is often limited to only cell lines or primary cells that have been rigorously purified. In the recent years, small desktop image-based cell counters have been developed for rapid cell concentration and viability measurement due [...]

By Channou Aing|2021-03-25T19:22:36+00:00March 25th, 2021|0 Comments

Journal of Microbiological Methods 2012

Read more Rapid Quantification of Pathogenic Fungi by Cellometer Image-Based Cytometry Journal of Microbiological Methods, September 2012, Berkes CA, Chan LL, Wilkinson A, Paradis B The objective of this study was to develop an image-based cytometric methodology for the quantification of viable pathogenic yeasts, which can offer increased sensitivity and efficiency when compared to the traditional colony forming unit (CFU) assay. Live/dead yeast quantification by flow cytometry has been previously demonstrated, however, adoption of flow cytometric detection of pathogenic yeasts has been limited for a number of practical reasons including its high cost and biosafety considerations. Our studies focus on detection [...]

By Channou Aing|2021-03-31T00:49:21+00:00March 25th, 2021|0 Comments

About Channou Aing