Channou Aing, Author at Nexcelom Bioscience

Poster: Celigo for measuring cell migration in wound healing assay

Download the Poster A Novel image based cytometry analysis for measuring cell migration in wound healing assay Leo L. Chan, Scott Cribbes, Sarah Kessel, Olivier Dery, Dmitry Kuksin, and Jean Qiu Cell migration is a multi-stepped, highly complex process that is involved in normal processes of cell proliferation and homeostasis, but also is exaggerated in the pathologies of metastasis and tumour invasion. Thecoordination of events has been studied at the molecular, biochemical and biophysical level for nearly 50 years. One assay which has been used throughout is the wound-healing or scratch assay. Simply defined, amonolayer of cells are grown and [...]

By Channou Aing|2021-03-31T00:48:39+00:00March 31st, 2021|0 Comments

Poster: Cell Analysis Method for Cell Cycle and Apoptosis using Celigo

Download the Poster A High Throughput Direct Adherent Cell Analysis Method for Cell Cycle and Apoptosis using Celigo Imaging Cytometer Leo L. Chan, Scott Cribbes, Sarah Kessel, Olivier Dery, Dmitry Kuksin, and Jean Qiu Apoptosis and cell cycle play an important role in various aspects of preclinical pharmaceutical drug discovery and validation. The ability to quickly determine the cytotoxic effect of chemical compounds on cancer cellsallows researchers to efficiently identify potential drug candidates for further development in the pharmaceutical discovery pipeline. Recently, a plate-based imaging cytometry system, Celigo Imaging Cytometer, has beenused to for high-throughput fluorescence cell cycle and apoptosis [...]

By Channou Aing|2021-03-31T00:45:49+00:00March 31st, 2021|0 Comments

Poster: A Simple Cost Effective Treatment for Declumping Super Yeast

Download the Poster A Simple Cost Effective Treatment for Declumping Super Yeast Leo L. Chan, Ashley Wilson, Kelsey McCulley, and Belen Belete-Gilbert In the early fermentation stages, many types of yeast cells form clusters that can increase the difficulty of both manual cell counting and automated image-based cell counting. In this work, we demonstrate a simple and cost effective method for declumping yeast cells in order to facilitate accurate cell counting and viability measurements. We examined the WLP090 San Diego Super Yeast which is characterized by high clumping during fermentation. A beer sample with Super Yeast was collected from the [...]

By Channou Aing|2021-03-31T00:46:00+00:00March 31st, 2021|0 Comments

Poster: CAR T cell concentration and viability using Cellometer

Download the Poster Consistently and accurately measure CAR T cell concentration and viability using Cellometer Image Cytometry for adoptive cell transfer therapy Dmitry Kuksin, Scott McMenemy, Scott Cribbes, Leo L. Chan Cancer immunotherapy research consists of multiple strategies that are aimed at enhancing immune responses, such as vaccine, antibody, immune checkpoint inhibitor, and adoptive cell transfer (ACT) therapies. Specifically for ACT therapy, it focuses on utilizing patient’s own cells for therapeutic treatment. Chimeric-antigen receptor (CAR) has become a major player in ACT, where T cells can be genetically engineered to express tumor-associated antigen using the Sleeping Beauty transposon system. In [...]

By Channou Aing|2021-03-31T00:39:25+00:00March 30th, 2021|0 Comments

Poster: Celigo detection of PBMC Mediated Cytotoxicity

Download the Poster A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity Leo L. Chan, Kelsey J, McCulley, Pinaki P. Banerjee Cell-mediated cytotoxicity assays have been an important functional test for investigating the cytotoxic effect of immune effector on target cancer cells. Cytotoxicity assays have traditionally been performed using the51Chromium (51Cr) release assay, which involves labeling the tumor cells (target) with radioisotopes. When the target cells are lysed by the immune cells (effector), they release the entrapped radioactive 51Chromium, which is measured to determine the level cytotoxicity induced by the effector cells. The [...]

By Channou Aing|2021-03-31T00:39:41+00:00March 30th, 2021|0 Comments

Poster: Celigo Real-time Capase 3/7 measurement of suspension and adherent cells

Download the Poster Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo image cytometer Surekha Bonasu, Sarah Kessel, Leo L Chan, Jean Qiu, Dmitry Kuksin A novel assay has been developed for real-time staining and detection of active caspases 3 and 7 in apoptotic cells using the Celigo, a micro-well imaging system. Traditionally, this type of apoptosis assay is an end point assay and is performed using fluorescently labeled Annexin-V. However, by coupling this image cytometry system (Celigo) developed by Nexcelom Bioscience LLC (Lawrence, MA) with live-cell dye for caspase 3/7 we are able to image and [...]

By Channou Aing|2021-03-31T00:39:59+00:00March 30th, 2021|0 Comments

Poster: Celigo comparison to CellTiter-Glo

Download the Poster Comparison of label-free cell cytotoxicity image cytometric detection method to CellTiter-Glo® Roopal Patel, Olivier Déry, Leo Li-Ying Chan, and Gina Wei Traditionally, cell proliferation has been assessed using detection methods such as CellTiter-Glo® or MTT assay. However, these methods require multiple steps, expensive reagents, and are fixed end-point assays, prohibiting the same cells from being repeatedly assessed over a time course.There has been a need for plate-based label-free cell proliferation and cytotoxicity detection method to directly determine the number of cells per well in order to eliminate the issues with the traditional methods. In this work, we [...]

By Channou Aing|2023-07-13T16:13:19+00:00March 30th, 2021|0 Comments

Poster: Celigo for T cell-mediated cytotoxicity of 3D tumor spheroids

Download the Poster A novel image cytometric analysis method for T cell-mediated cytotoxicity of 3D tumor spheroids Leo Li-Ying Chan, Laure Humbert, Sarah Kessel, and Scott Cribbes Cell-mediated cytotoxicity assays have been frequently performed to characterize cancer cytotoxic potential of immune cells, antibodies, and drug compounds. Traditionally, these assays are performed using release assays such as Cr 51 (radioactivity), Calcein (fluorescence), or LDH (enzymatic). However, release assays have limitations such as the handling of hazardous material, the indirect measurement of cell death leading to an under estimation of cytotoxicity, the requirement for a large volume of cell sample, and the [...]

By Channou Aing|2021-03-31T00:40:19+00:00March 30th, 2021|0 Comments

Poster: Celigo 3D tumor spheroid screening method for drug discovery

Download the Poster A high-throughput 3D tumor spheroid screening method for drug discovery using imaging cytometry Sarah Kessel, Scott Cribbes, Olivier Déry, and Leo Li-Ying Chan There have been 1600 FDA approved drugs since their inception in 1938. Currently it costs approximately 883 million dollars to bring a drug candidate to the market, and often times the potential candidate fails at the beginning of the clinical phase. Therefore, it is important to develop drug screening methods that are more clinically relevant or predictable. The current 2D methods for cancer drug discovery have had some difficulty in identifying potential drug candidates [...]

By Channou Aing|2021-03-31T00:40:33+00:00March 30th, 2021|0 Comments

Poster: Cellometer X2 viability validation method

Download the Poster A rapid viability validation method of flash pasteurized yeast using Cellometer image cytometer Jaime Schier, Joseph Thomas, Dmitry Kuksin, and Leo L. Chan Flash pasteurization has traditionally been deployed in the brewing industry to stabilize beer by reducing the number of beer spoilage organisms per ml, ensuring long and stable shelf life free from defective flavorsand aromas created by the likes of L. brevis and P. damnosus. As innovative new beers from the craft segment arrive, new challenges to beer stability sometimes manifest. In the case of beers containing high levels of residual fermentable sugar, a new [...]

By Channou Aing|2021-03-31T00:41:27+00:00March 30th, 2021|0 Comments

About Channou Aing