https://youtu.be/LNTfoX2OJ9I Video: How to set up an Antibody Binding Assay on your Celigo Sarah Kessel shows how to perform assay design and development for Antibody Binding assay, including appropriate controls, on Celigo image cytometer.
https://youtu.be/FoH7u5GNpp8 Video: Getting Better Acquainted with the Celigo Gating Tab Sarah Kessel goes over the different buttons and features in the gate tab, especially how to create graphs and subpopulations, similar to flow-like gating.
https://youtu.be/4OmQh_i7fNA Video: Getting to know the Analyze Tab Parameters on Celigo Sarah Kessel goes over how image analysis is done on Celigo, especially what are image algorithms, intensity threshold, pixel intensity and more.
https://youtu.be/LqUnAy_6-7U Video: Celigo Viability Assays with Hoechst & PI Sarah Kessel demonstrates a basic cell health viability assay on Celigo cytometer with reagents Hoechst and PI.
https://youtu.be/UJzsUk__sZE Video: How to Run a High Throughput Viability Assay on Celigo Suspension Cells using AO/PI Stain Sarah Kessel discuss how to perform a high-throughput viability assay using AOPI. Topics include plate preparation, imaging and analysis on Celigo, calculations and evaluate data, Sample plate layout, and cell concentration calculation.
https://youtu.be/dQr4_ZzfTHI Video: How to run a High-Throughput Antibody Internalization Assay with pHrodo Sarah Kessel shows how to perform assay design and development for Antibody Internalization assay with pHrodo, including appropriate controls, on Celigo image cytometer.
https://youtu.be/pQBv2XGGOsg Video: How to Quickly set up a 6-well plate for Monitoring your GFP-Transfected Cells on Celigo Sarah Kessel demonstrates an easier and faster way to image and analyze a 6-well GFP transfection assay, utilizing sub-sampling prior to imaging the whole plate
https://youtu.be/mJCRok204nM Video: Better Organize and Manage your Data on Celigo Sarah Kessel discuss data management features and best practices for your Celigo images.
https://youtu.be/b2B0uD96XbA Video: How to Monitor 3D Multicellular Tumor Spheroid Invasion and Migration into Extracellular Matrix Topics include Assay setup, Image acquisition, Image analysis, Viewing of kinetic images
https://youtu.be/kIyQUhtd5_w Video: How to Run an Apoptosis Assay on the Celigo Using Annexin V PI Hoechst Sarah Kessel shows how to prepare an apoptosis assay using Annexin V, PI and Hoechst, then how to acquire and analyze images on Celigo for a fast direct cell count evaluation of cell health in a plate based method.