Transfection/Transduction
Benefits of Transduced fibroblasts acquired on Celigo Cell Imaging Cytometer
- Rapid, whole-well analysis of transfected/transduced cultures (1536-well to 6-well)
- Quickly identify optimal parameters for high-efficiency transfection & transduction
- Determine transient and stable transfection/transduction rates using live imaging
- Reliable analysis of many different adherent and non-adherent cell types
Introduction
Optimization of cellular transfection and transduction includes choosing a protocol, determining the appropriate mass of plasmid/virus, and evaluating the optimum time after transfection/transduction for the best expression of the construct of interest. Using fluorescent reporters and non-destructive in situ imaging on Celigo cell imaging cytometer, these parameters can be rapidly optimized by repeated imaging of one set of wells or flasks over a period of time. Accurate whole-well bright field cell counting generates cell-based data normalized to absolute cell numbers.
GFP Transfection Optimization in 96-well Plates without Trypsinization
Fluorescent Proteins
- Identifies cells in bright field image
- Measures fluorescent protein signals in green and red channels
- Label-free, non-invasive – no need to trypsinize adherence cells
- Quantifies fluorescent protein signals on a cell-by-cell basis repeatedly on the same plate providing temporal data
- Add propidium iodide for viability of GFP transfect cells in the same well
Imaged and counted cells
Monitoring transfection rates and viability over 5 days
<h2″>Transduced fibroblasts acquired on Celigo Cell Imaging Cytometer