Tumorsphere Formation & Clonogenic Survival
Live cell analysis of tumorsphere formation and clonogenic assays to quantify size and number of formed tumorspheres.
- Directly image tumor spheroids in various microwell formats
- Non-invasive bright field imaging allows the user to image the same plate over multiple days
- Perform a two-color fluorescent viability assay
Introduction
The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.
Image and Quantify Tumorsphere Formation and Clonogenic Survival Assays
Formation and Counting of Tumorspheres from Various Cell Lines
Quantification of Methylene Blue-Stained Clonogenic Colonies in 6-well Plates
Whole-well image of the control sample
Whole-well image and counted colonies of siRNA treated sample
Colony number of MDA MB468 cells following siRNA knockdown