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Cellometer Features and Applications Cellometer Auto T4 / M10Cellometer Auto X4Cellometer Vision
Features Auto T4 / M10
Automated Cell Counter		 Auto X4 Fluorescence Assisted Cell Counter Vision Fluorescence Cell Analysis
  T4M10 Ideal for automating
manual cell counting:
• Cell lines
• Viability w/trypan blue
 X4 Ideal for automating
manual cell counting:
• Cell lines
• Viability w/trypan blue
• Primary cells/messy samples
• PI viability
 Vision A versatile research tool to
perform a variety of cell
counting and analysis assays:
• Cell lines or primary cells
• Viability w/trypan blue, or PI
• Fluorescence analysis: GFP,dual staining, etc.
Functionality
Brightfield Imaging
•
•
•
Fluorescence Imaging
•
Fluoresence Channels
1
2
Intensity Measurements
•
Low Signal Detection
•
Magnification Options
Standard
•
•
•
10X
• (Auto M10)
•
Assays†
Cell Line – total cell concentration + trypan blue viability
•
•
•
Cell Line – total cell concentration + trypan blue viability
•
•
GFP**Transfection efficiency – total cell, GFP positive cell
concentrations, transfection efficiency, fluorescence intensity
•
Cell Line – nucleated cell concentration (stained by DAPI, AO, etc.)
•
•
PBMC (clean preparation)
•
•
•
PBMC (with RBC and platelets present) – concentration
•
•
PBMC (with RBC and platelets present) – live cell concentration
•
•
PBMC (with RBC and platelets present) – live / dead cell concentration + viability
•
WBC in whole blood w/o lysing – WBC concentration
•
•
Primary cells*** (without lysing) – concentration
•
•
Primary cells (without lysing) – live cell concentration
•
•
Primary cells (without lysing) – live / dead concentration + viability
•
Digested tissues – live / dead concentration + viability
•
Apoptotic cell counting
•
•
Primary hepatocyte – live / dead concentration + viability
•
Fresh primary adipocyte – cell concentration, size histogram
•
•
Nuclei – concentration distribution
•
•
Beads – concentration and size
•
•
•
Beads – concentration, size and fluorescence
•
•
Yeast (clean without debris) – concentration
• (Auto M10)
•
Yeast – live cell concentration + viability
•
Algae (clean without debris) – concentration
• (Auto M10)
•
Algae (with debris) – concentration
•
Surface markers and other low fluorescence – cell concentration and fluorescence
•
Cells stained with two fluorescent dyes – concentrations
•
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 * Also used are fluorescent nucleic acid staining dyes, such as EB, 7-AAD, Fluorogenic intracellular esterase substrates, such as fluorescein diacetate (FDA), CalceinAM
** Including other fluorescent proteins, such as DsRed (RFP), YFP, CFP
*** Primary cell examples include spleenocyte, bone marrow, cord blood, BAL (bronchoalveolar lavage)
†Cellometer cell counters are used for many different fluorescent assays. The list is rapidly expanding; the table above represents only a partial list of commonly used assays.
If you have new assays not listed here, please contact our applications specialists for the most current information.