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Cellometer Auto X4 Applications

Cell Lines: Cell Concentration

293

293 293

CHO

CHO CHO

L929

L929 L929

Mouse RBC

Mouse RBC Mouse RBC

Neuroblastoma

Neuroblastoma Neuroblastoma

PEC

PEC PEC
Sample Name Cell Count Dilution Factor Concentration Mean Diameter
293
372
1
6.65E+05
17.5
CHO
436
1
7.65E+05
11.5
L929
542
1
9.58E+05
13.8
Mouse RBC
3158
1
5.46E+06
6.7
Neuroblastoma
103
1
1.82E+05
13.8
PEC
942
1
1.63E+06
7.4

Cell Lines: Live Cell Concentration and Trypan Blue Viability

293 Trypan Blue

293 Trypan Blue 293 Trypan Blue

Hela Trypan Blue

Hela Trypan Blue Hela Trypan Blue
Sample Name Count Type Cell Count Dilution Factor Concentration Viability
Cultured stem cells+trypan
Cultured stem cells+trypan
Live
Trypan
445
30
2
2
1.57E+06
1.06E+05
93.70%
HeLa+trypan
HeLa+trypan
Live
Trypan
209
438
2
2
7.37E+05
1.55E+06
32.30%
HL60+trypan blue
HL60+trypan blue
Live
Trypan
160
19
2
2
5.65E+05
6.71E+04
89.40%

Primary Cells: Total Nucleated Cell Concentration Stained with AO

BAL

BAL BAL
BAL

Cord Blood

Cord Blood Cord Blood
Cord Blood

Human WBC in Unlysed Whole Blood

Human WBC in unlysed whole blood Human WBC in Unlysed Whole Blood
Human WBC in Unlysed Whole Blood

Human PBMC

Human PBMC Human PBMC
Human PBMC

Mouse Spleenocyte

Mouse Spleenocyte Mouse Spleenocyte
Mouse Spleenocyte
Sample Name Cell Count Dilution Factor Concentration
PBMC+ AO
308
2
1.07E+06
Human cord blood
806
2
2.80E+06
Human WBC+ AO
220
10
3.85E+06

Live Cell Concentration and PI Viability

SKBr3

SKBr3 SKBr3
SKBr3 SKBr3

Cell Stained with DAPI

SKBr3

SKBr3 SKBr3

Cell Stained with Horeschet

SKBr3 Horeschet

SKBr3 Horeschet SKBr3 Horeschet

Cell Concentration and Viability by Fluorescein Diacetate(FDA) Hydrolysis

T47D

T47D T47D
T47D T47D