Stem Cells, Primary Cells, Cell Line
Figure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample |
N Value |
Average Live Cell Concentration |
% Viability |
CV of Concentration |
CV of Viability |
|---|---|---|---|---|---|
A |
4 |
4.10E+06 |
78 |
1% |
2% |
B |
4 |
0.0025E+06 |
0.6 |
N/A |
N/A |
C |
4 |
2.48E+06 |
36 |
4% |
7% |
Figure 1: Table of results for AOPI viability using the Cellometer Auto 2000.
The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat cells
using AOPI for cell viability. Four measurements were performed for each sample. The viability average was calculated
and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell
concentration and viability of mammalian cells.
Cell Line Viability using Propidium Iodide Only
Figure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample |
N Value |
Average Live Cell Concentration |
% Viability |
CV of Concentration |
CV of Viability |
|---|---|---|---|---|---|
A |
4 |
4.20E+06 |
91.1 |
10% |
2% |
B |
4 |
1.06E+06 |
22.7 |
7% |
1% |
C |
4 |
3.27E+06 |
57.5 |
7% |
7% |
Figure 1: Table of results for cell viability using PI only.
The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat
cells using PI for cell viability. Four measurements were performed for each sample. The viability average was
calculated and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000
in measuring cell concentration and viability of mammalian cells.
Cell Line Total Cell Concentration
Figure 2: Jurkat cell concentrations at various sample dilution factors. N=4. Error bars correspond to the Standard Deviations
for cell concentration.
Sample |
Dilution Factor |
Average Cell Concentration |
CV of Concentration |
Standard Deviation |
N Value |
|---|---|---|---|---|---|
A |
1 |
5.80E+06 |
1% |
4.8E+04 |
4 |
B |
2 |
3.39E+06 |
2% |
8.12E+04 |
4 |
C |
3 |
1.63E+06 |
6% |
9.54E+04 |
4 |
D |
4 |
8.27E+05 |
6% |
5.33E+04 |
4 |
Figure 1: Table of results for total cell concentration for the Cellometer Auto 2000 instrument.
The results indicate the accuracy of the Cellometer Auto 2000 instrument cell concentration. Serial
dilutions (2-, 4-, and 8-fold) were prepared using PBS and Jurkat cells. Four measurements were performed for
each sample and the averages were calculated and plotted. A linear correlation between the
concentration measurements and 1/dilution factor was observed. The results show the reliability and accuracy
of the Cellometer Auto 2000 in measuring cell concentration.
Low Concentration of Cells using Acridine Orange
Figure 2: Graph of Concentration for 4 different measurements using the Low Concentration of Cells Assay and AO on the Cellometer Auto 2000.
Low Concentration AO |
F1 Concentration |
|---|---|
Sample 1 |
5.91E+04 |
Sample 2 |
6.40E+04 |
Sample 3 |
6.58E+04 |
Sample 4 |
6.90E+04 |
Average |
6.45E+04 |
Stdev |
4.14E+03 |
CV |
6% |
Figure 1: Table of results from the Low Concentration of Cells assay.
The results indicate the accuracy of the Cellometer Auto 2000 instrument when counting Jurkat cells at
very low concentrations (between 5-7E+04 cells/ml). AO was used to measure the concentration of cells in the F1
channel. Results from 4 measurements were plotted. The CV values are very close, which indicates the
consistency of the measurements. The results show the reliability and accuracy of the Cellometer Auto 2000 in
measuring at low cell concentration.
Cellometer Videos
Cell Counting Tutorials
Cellometer Auto 2000 Resources
