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Demo Cellometer Vision...

At Booth #1181 in Baltimore, MD October 9th-12th

The fastest, most reliable advanced counting method for counting and determining viability of stem cells, and direct counts of WBCs from peripheral blood samples without lysing RBCs.
Innovative, patent pending counting software to decluster cell clumps.
Dual fluorescence staining for distinctive live/dead cell imaging.

Stem Cell Count and Viability	Cell Proliferation Assay	Apoptosis	Direct Counting WBC from Whole Blood
PI Viability	Cell Killing Assay	Vaccine	PBMC Counting and Viability
Quantify GFP Transfection Rates

Direct Count of White Blood Cells from Peripheral Blood Sample without Lysing Red Blood Cells

Cellometer Vision

Cellometer Auto X4

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Introduction

Brightfield image of whole blood.
WBC's are not visible amongst RBC's

Fluorescent image showing AO stained WBC's

Fluorescent image showing AO
stained WBC's. RBC's are not visible.

Cellometer Vision incorporates image based cell counting
and fluorescence detection in a compact and easy-to-use
instrument. With fluorescence detection capabilities,
Cellometer Vision is an ideal solution for many complex cell population characterization assays such as rapidly counting white-blood-cells in whole blood.

As one of the most important cellular components in peripheral blood, the concentration of white blood cells is routinely used in researching various diseases, such as bacteria/virus infections, leukemia, or leucopenia. However, conventional methods to count white blood cells include a time consuming and tedious RBC lysis process.

Vision can be used to simplify traditional methods by mixing a cell membrane permeable DNA dye (acridine orange) with diluted whole blood sample that specifically stains white blood cells. To quantitatively analyze WBCs, we simply pipette 20ml of treated sample into a Cellometer Disposable Counting Chamber and place the chamber into the instrument. Since the counting chamber is disposable, no washing is required between samples, there is no risk of cross contamination of different samples, and the risk of exposure to biohazard materials is reduced.

Using proprietary algorithms, Cellometer Vision's robust operating software accurately analyzes cell images, and generates counting data in less than 60 seconds typically. Cell images and all analysis data, including cell size distribution histograms, can be saved for documentation. Data can also be easily exported to Microsoft Excel spreadsheets for further analysis.

Counting results box displays fluorescence cell count & concentration.

Method

Direct AO labeling of white blood cells:

Collect 1 drop of peripheral blood sample (20-50µl).
Take 20µl of blood sample and dilute the sample with 180µl of PBS.
Apply 5µl of Acridine Orange solution to 100µl diluted
blood sample (final concentration 2-10µg/ml).

Running Assay:

Load 20µl of sample into the disposable counting chamber
Insert chamber into Cellometer Vision
Select Assay from drop-down menu
Enter Sample ID for this sample
Preview cell images and click 'Count' to begin analyzing sample
Review images and counting data.
Save or export images and/or report data.

Step 3: Choose Assay from drop down menu

Step 4: Enter Sample ID

Results

AO Stained WBC’s are clearly visible in the acquired fluorescence image (Figure 1). The software indicates counted cells with a green circle (enlarged to show detail). Results display indicates fluorescence cells counted and automatically calculates concentration.

Figure 1. Fluorescent image showing counted AO
stained WBC's. RBC's are not visible.

Cell and fluorescent size distribution histograms, scatter plots and data files can also be instantly generated. All experimental data can be instantly transferred to an Excel spreadsheet (Figure 2) or saved in a data table (Figure 3).